Analysis of microarchitectural changes in a mouse temporomandibular joint osteoarthritis model.
Analysis of microarchitectural changes in a mouse temporomandibular joint osteoarthritis model.
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DOI：
10.1016/j.archoralbio.2009.10.001
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发表时间：
2009-12
影响因子：
3
通讯作者：
Wadhwa, S.
中科院分区：
文献类型：
--
作者：
Chen, J.;Gupta, T.;Barasz, J. A.;Kalajzic, Z.;Yeh, W. -C.;Drissi, H.;Hand, A. R.;Wadhwa, S.
对于影响美国约1%人口的颞下颌关节(TMJ)骨关节炎(OA)的自然病程，人们知之甚少。本研究的目的是观察Biglycan/Fmod(BGN/Fmod)双缺陷小鼠在6个月时发生TMJ-OA时，髁状突软骨和软骨下骨的早期微结构和分子变化。对3月龄(44只)、9月龄(52只)、野生型(46只)和BGN/Fmod(50只)双缺陷小鼠的TMJ进行检测。对24例软骨下骨进行了显微CT分析，26例进行了关节突软骨纤维直径的透射电子显微镜观察，45例进行了骨和软骨成熟标志物基因表达的实时定量聚合酶链式反应(RT-PCR)分析。与WT对照组相比，BGN/Fmod双缺陷3个月组小鼠下颌骨软骨胶原纤维直径显著增大，甲状旁腺相关蛋白表达显著降低。9月龄BGN/Fmod双缺陷小鼠骨体积和软骨下骨总体积增加，下颌骨髁头X型胶原和聚集素表达增加。我们发现，在BGN/Fmod双缺陷小鼠的TMJ中，在骨关节炎过程中，关节突软骨微结构的变化先于软骨下骨的变化。
Little is known about the natural progression of the disease process of temporomandibular joint (TMJ) osteoarthritis (OA), which affects approximately 1 % of the US population. The goal of this study was to examine the early microarchitectural and molecular changes in the condylar cartilage and subchondral bone in biglycan/fibromodulin (Bgn/Fmod) double-deficient mice, which develop TMJ-OA at 6 months. TMJs from 3 month old (n=44) and 9 month old (n=52) wild-type (WT n=46) and Bgn/Fmod (n=50) double-deficient mice were evaluated. Micro-CT analysis of the subchondral bone (n=24), transmission electron microscopy for condylar cartilage fibril diameters (n=26), and real time PCR analysis for gene expression for bone and cartilage maturation markers (n=45) was performed. A statistically significant increase in collagen fibril diameter of the condylar cartilage and a decrease in expression of Parathyroid related protein in the mandibular condylar head were observed in the 3 month Bgn/Fmod double-deficient mice compared to WT controls. The 9 month Bgn/Fmod double-deficient mouse demonstrated an increase in bone volume and total volume in subchondral bone, and an increase in the expression of Collagen Type X and Aggrecan in the mandibular condylar head compared to the WT controls. We found that changes in the microarchitecture of the condylar cartilage preceded changes in the subchondral bone during OA in the TMJ in Bgn/Fmod double-deficient mice.
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作者：
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