Impact of route of exposure and challenge dose on the pathogenesis of H7N9 low pathogenicity avian influenza virus in chickens.
Impact of route of exposure and challenge dose on the pathogenesis of H7N9 low pathogenicity avian influenza virus in chickens.
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DOI：
10.1016/j.virol.2015.01.013
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发表时间：
2015-03
期刊：
影响因子：
3.7
通讯作者：
Kapczynski, Darrell R.
中科院分区：
文献类型：
--
作者：
Spackman, Erica;Pantin-Jackwood, Mary;Swayne, David E.;Suarez, David L.;Kapczynski, Darrell R.
H7N9甲型流感于2013年初在中国首次引起人类感染。病毒遗传学、患者接触家禽的历史以及以前的实验研究表明，该病毒的来源是一种家禽，如鸡。为了更好地了解H7N9在鸡体内的生态环境，我们对白来角鸡（表蛋鸡）和白普利茅斯石鸡（肉鸡）这两个常见品种的A/Anhui/1/2013型H7N9进行了感染剂量和发病机制评估。上呼吸道给药剂量为106或108 EID50时，未观察到每只鸡的发病或死亡，平均感染剂量（106 EID50）高于预期，表明该病毒对鸡的适应性较差。在经静脉注射途径接种的鸡中，病毒以较高滴度脱落并传播到肾脏。对另外三种人类源性H7N9病毒的挑战实验显示出类似的病毒复制模式。
H7N9 influenza A first caused human infections in early 2013 in China. Virus genetics, histories of patient exposures to poultry, and previous experimental studies suggest the source of the virus is a domestic avian species, such as chickens. In order to better understand the ecology of this H7N9 in chickens, we evaluated the infectious dose and pathogenesis of A/Anhui/1/2013 H7N9 in two common breeds of chickens, White Leghorns (table-egg layers) and White Plymouth Rocks (meat chickens). No morbidity or mortality were observed with doses of 106 or 108 EID50 per bird when administered by the upper-respiratory route, and the mean infectious dose (106 EID50) was higher than expected, suggesting that the virus is poorly adapted to chickens. Virus was shed at higher titers and spread to the kidneys in chickens inoculated by the intravenous route. Challenge experiments with three other human-origin H7N9 viruses showed a similar pattern of virus replication.
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