Leveraging Atriplex hortensis choline monooxygenase to improve chilling tolerance in cotton
Leveraging Atriplex hortensis choline monooxygenase to improve chilling tolerance in cotton
复制标题
利用滨藜胆碱单加氧酶提高棉花的耐冷性
DOI：
10.1016/j.envexpbot.2019.03.012
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发表时间：
2019-06
影响因子：
5.7
通讯作者：
ui Guo
中科院分区：
文献类型：
--
作者：
Yanan Wang;Chengzhen Liang;ZhigangMeng;Yanyan Li;Muhammad Ali Abid;Muhammad Askari;Peilin Wang;Yuan Wang;Guoqing Sun;Yongping Cai;Shou-Yi Chen;Yi Lin;Rui Zhang;S;ui Guo
低温是限制棉花苗期生长和产量的主要因素。然而，提高耐冷性通常与农业生产中的产量呈负相关。在这里，我们证明了转基因棉花表达Atriplex hortensis胆碱单加氧酶（AhCMO）大大提高了抗冷胁迫。抗冷性的提高主要是由于甜菜碱和脯氨酸等植物保护物质含量的增加。通过RNA测序的全基因组表达谱分析，在分子水平上进一步验证了耐冷性的提高。进一步的分析表明，低温处理后转AhCMO基因棉花与野生型相比，参与活性氧（ROS）清除的基因数目下调，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性降低。更重要的是，在正常生长条件下，AhCMO在棉花中的过表达适度提高了棉花纤维产量。说明转AhCMO基因棉花通过直接积累细胞内的植物保护物质来提高其耐低温性。利用生物技术手段调控AhCMO基因的表达是提高棉花耐冷性的有效途径。
Low temperature is a major factor limiting seedling growth and the production of cotton (Gossypium hirsutumL.). However, enhancing chilling tolerance is typically negatively correlated with yield in agricultural production. Here, we demonstrate that transgenic cotton expressingAtriplex hortensis choline monooxygenase(AhCMO) greatly enhanced resistance to chilling stress. The promotion of chilling tolerance is mainly due to an increase in the content of osmoprotectants, especially glycine betaine and proline. The increased chilling tolerance was further verified at the molecular level using genome-wide expression profiling by RNA-sequencing. Further detailed analysis showed that the number of genes involved in scavenging of reactive oxygen species (ROS) was down-regulated and the activity of superoxide dismutase (SOD) and catalase (CAT) were decreased inAhCMOtransgenic cotton compared with wild type after low temperture treatment. More importantly, overexpression ofAhCMOin cotton moderately improved cotton fiber yield in normal growth condition. These data show thatAhCMOtransgenic cotton enhances low temperature tolerance via directly accumulating cellular osmoprotectants. Manipulating the expression ofAhCMOby biotechnological tools could be a powerful method to enhance chilling tolerance in cotton.
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影响因子：
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