An Alfin-like gene from Atriplex hortensis enhances salt and drought tolerance and abscisic acid response in transgenic Arabidopsis.
An Alfin-like gene from Atriplex hortensis enhances salt and drought tolerance and abscisic acid response in transgenic Arabidopsis.
复制标题
DOI：
10.1038/s41598-018-21148-9
复制
发表时间：
2018-02-09
影响因子：
4.6
通讯作者：
Zhang JS
中科院分区：
文献类型：
--
作者：
Tao JJ;Wei W;Pan WJ;Lu L;Li QT;Ma JB;Zhang WK;Ma B;Chen SY;Zhang JS
Alfin-like （AL）是一个小型的植物特异性基因家族，在根系生长和非生物胁迫响应中发挥重要作用。在这里，我们的目的是从耐应力物种刺蒺藜（Atriplex hortensis）中鉴定出新的耐应力AL基因。我们一共分离了4个AhAL基因，它们都编码具有顺式元件结合和转抑制活性的核定位蛋白。拟南芥中AhAL1的组成性表达促进了植物在盐水条件下的生存，而其他三个AhAL基因的表达均导致盐过敏反应，表明AhAL家族的功能分化。与WT相比，AhAL1基因表达植株的抗旱性增强，表现为存活率提高、生长改善、丙二醛（MDA）含量降低、水分流失减少。此外，AhAL1基因表达植株的脱落酸（ABA）介导的气孔关闭、种子萌发抑制和初生根伸长增强。进一步分析表明，AhAL1可以结合GRF7、DREB1C和多个a组PP2C基因的启动子区域，抑制其表达。相应的，正应激调节基因DREB1A、DREB2A和3种abf在表达ahal1的植物中表达量均增加。基于这些结果，AhAL1被确定为提高作物非生物胁迫耐受性的新候选基因。
Alfin-like (AL) is a small plant-specific gene family with prominent roles in root growth and abiotic stress response. Here, we aimed to identify novel stress tolerance AL genes from the stress-tolerant species Atriplex hortensis. Totally, we isolated four AhAL genes, all encoding nuclear-localized proteins with cis-element-binding and transrepression activities. Constitutive expression of AhAL1 in Arabidopsis facilitated plants to survive under saline condition, while expressing anyone of the other three AhAL genes led to salt-hypersensitive response, indicating functional divergence of AhAL family. AhAL1 also conferred enhanced drought tolerance, as judged from enhanced survival, improved growth, decreased malonaldehyde (MDA) content and reduced water loss in AhAL1-expressing plants compared to WT. In addition, abscisic acid (ABA)-mediated stomatal closure and inhibition of seed germination and primary root elongation were enhanced in AhAL1-transgenic plants. Further analysis demonstrated that AhAL1 could bind to promoter regions of GRF7, DREB1C and several group-A PP2C genes and repress their expression. Correspondingly, the expression levels of positive stress regulator genes DREB1A, DREB2A and three ABFs were all increased in AhAL1-expressing plants. Based on these results, AhAL1 was identified as a novel candidate gene for improving abiotic stress tolerance of crop plants.
登录
查看更多内容
DOI：
10.1126/science.1173041
发表时间：
2009-05-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Park SY;Fung P;Nishimura N;Jensen DR;Fujii H;Zhao Y;Lumba S;Santiago J;Rodrigues A;Chow TF;Alfred SE;Bonetta D;Finkelstein R;Provart NJ;Desveaux D;Rodriguez PL;McCourt P;Zhu JK;Schroeder JI;Volkman BF;Cutler SR
通讯作者：
Cutler SR
影响因子：
7.2
作者：
Hao, Yu-Jun;Wei, Wei;Chen, Shou-Yi
通讯作者：
Chen, Shou-Yi
影响因子：
13.8
作者：
Ramu, Vemanna S.;Swetha, Thavarekere N.;Udayakumar, Makarla
通讯作者：
Udayakumar, Makarla
影响因子：
44.1
作者：
Liao, Yong;Zou, Hong-Feng;Chen, Shou-Yi
通讯作者：
Chen, Shou-Yi
影响因子：
7.2
作者：
Lee, Woo Yong;Lee, Daeyoup;Kwon, Chang Seob
通讯作者：
Kwon, Chang Seob