Symbiotic microbes affect the expression of male reproductive genes in Glossina m. morsitans.
Symbiotic microbes affect the expression of male reproductive genes in Glossina m. morsitans.
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DOI：
10.1186/s12866-018-1289-2
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发表时间：
2018-11-23
期刊：
影响因子：
4.2
通讯作者：
Malacrida AR
中科院分区：
文献类型：
--
作者：
Scolari F;Attardo GM;Aksoy E;Weiss B;Savini G;Takac P;Abd-Alla A;Parker AG;Aksoy S;Malacrida AR
采采蝇（Tsetse flies）是双翅目（Diptera）舌蝇科（Glossinidae）的一种重要的繁殖生物学类群。雌性通过腺营养胎生繁殖，滋养生长的幼虫进入它们的修改子宫，直到分娩。雄性将其精子和精液（由睾丸和雄性附腺产生）转移到交配时在雌性生殖道内短暂形成的精荚囊中。两性都是专性供血者，并与内共生体形成了紧密的关系，内共生体已经证明可以提供饮食中缺乏的必需营养素。然而，迄今为止，采采蝇及其共生体之间的伙伴关系仅在雌性中在分子、基因组和代谢组学水平上进行了研究，而微生物群在雄性生殖中的作用仍未被探索。在这里，我们开始解开微生物群对Glossina m的影响。morsitans（G. morsitans）雄性生殖生物学，通过母体抗生素治疗和膳食补充剂从被剥夺其内共生体（脱共生体）的雄性的生殖组织产生转录组。然后，我们比较了正常的雄性生殖道中表达的基因的转录谱和这些apossymbiotic苍蝇。我们发现，去除微生物群会通过抑制男性附腺（MAG）中基因的活性（包括编码精液蛋白的序列）和增加睾丸中基因的表达来影响几个男性生殖基因。特别是在MAG中，与交配、免疫和精液成分合成相关的基因的表达减少。在睾丸中，共生体的缺乏激活了男性生殖基础上的代谢装置中的基因，包括精子的产生，运动和功能。我们的研究结果反映了雄性附腺和睾丸在支持雄性生殖方面的互补作用，并为解开雄性昆虫和内共生体之间的相互作用开辟了新的途径。从应用的角度来看，解开采采蝇共生体和男性生殖生理之间的代谢和功能的关系，将提供有用的基本信息，了解生物学基础提高男性生殖成功的采采蝇。这一信息在通过昆虫不育技术（SIT）控制采采蝇种群及其对锥虫传播的影响方面特别重要。本文的在线版本（10.1186/s12866-018-1289-2）包含补充材料，可供授权用户使用。
Tsetse flies (Diptera, Glossinidae) display unique reproductive biology traits. Females reproduce through adenotrophic viviparity, nourishing the growing larva into their modified uterus until parturition. Males transfer their sperm and seminal fluid, produced by both testes and male accessory glands, in a spermatophore capsule transiently formed within the female reproductive tract upon mating. Both sexes are obligate blood feeders and have evolved tight relationships with endosymbionts, already shown to provide essential nutrients lacking in their diet. However, the partnership between tsetse and its symbionts has so far been investigated, at the molecular, genomic and metabolomics level, only in females, whereas the roles of microbiota in male reproduction are still unexplored. Here we begin unravelling the impact of microbiota on Glossina m. morsitans (G. morsitans) male reproductive biology by generating transcriptomes from the reproductive tissues of males deprived of their endosymbionts (aposymbiotic) via maternal antibiotic treatment and dietary supplementation. We then compared the transcriptional profiles of genes expressed in the male reproductive tract of normal and these aposymbiotic flies. We showed that microbiota removal impacts several male reproductive genes by depressing the activity of genes in the male accessory glands (MAGs), including sequences encoding seminal fluid proteins, and increasing expression of genes in the testes. In the MAGs, in particular, the expression of genes related to mating, immunity and seminal fluid components’ synthesis is reduced. In the testes, the absence of symbionts activates genes involved in the metabolic apparatus at the basis of male reproduction, including sperm production, motility and function. Our findings mirrored the complementary roles male accessory glands and testes play in supporting male reproduction and open new avenues for disentangling the interplay between male insects and endosymbionts. From an applied perspective, unravelling the metabolic and functional relationships between tsetse symbionts and male reproductive physiology will provide fundamental information useful to understanding the biology underlying improved male reproductive success in tsetse. This information is of particular importance in the context of tsetse population control via Sterile Insect Technique (SIT) and its impact on trypanosomiasis transmission. The online version of this article (10.1186/s12866-018-1289-2) contains supplementary material, which is available to authorized users.
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影响因子：
23.8
作者：
Douglas AE
通讯作者：
Douglas AE
影响因子：
30.8
作者：
Akman, L;Yamashita, A;Aksoy, S
通讯作者：
Aksoy, S
影响因子：
2.2
作者：
Attardo, Geoffrey M.;Lohs, Claudia;Heddi, Abdelaziz;Alam, Uzma H.;Yildirim, Suleyman;Aksoy, Serap
通讯作者：
Aksoy, Serap
影响因子：
12.3
作者：
Conesa A;Madrigal P;Tarazona S;Gomez-Cabrero D;Cervera A;McPherson A;Szcześniak MW;Gaffney DJ;Elo LL;Zhang X;Mortazavi A
通讯作者：
Mortazavi A
影响因子：
3.3
作者：
Fiumera, Anthony C.;Dumont, Bethany L.;Clark, Andrew G.
通讯作者：
Clark, Andrew G.