The time course of activity-regulated cytoskeletal (ARC) gene and protein expression in the whisker-barrel circuit using two paradigms of whisker stimulation.
The time course of activity-regulated cytoskeletal (ARC) gene and protein expression in the whisker-barrel circuit using two paradigms of whisker stimulation.
复制标题
使用两个晶须刺激范式在晶须桶回路中，活性调节的细胞骨架（ARC）基因和蛋白表达的时间过程。
DOI：
10.1016/j.bbr.2015.01.032
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发表时间：
2015-05-01
影响因子：
2.7
通讯作者：
Thomas TC
中科院分区：
文献类型：
--
作者：
Khodadad A;Adelson PD;Lifshitz J;Thomas TC
Immediate early genes have previously demonstrated a rapid increase in gene expression after various behavioral paradigms. The main focus of this article is to identify a molecular marker of circuit activation after manual whisker stimulation or exploration of a novel environment. To this end, we investigated the dynamics of ARC transcription in adult male rats during whisker somatosensation throughout the whisker barrel circuit. At various time points, tissue was biopsied from the ventral posterior medial nucleus (VPM) of the thalamus, primary somatosensory barrel field (S1BF) cortex and hippocampus for quantification using real-time PCR and western blot. Our results show that there were no significant differences in ARC gene or protein expression in the VPM after both types of stimulation. However, manual whisker stimulation resulted in increased ARC gene expression at 15, 30, 60 and 300 minutes in the S1BF, and 15 minutes in the hippocampus (p<0.05). Also, exploration of a novel environment resulted in increased ARC mRNA expression at 15 and 30 minutes in the S1BF and at 15 minutes in the hippocampus (p<0.05). The type of stimulation (manual versus exploration of a novel environment) influenced the magnitude of ARC gene expression in the S1BF (p<0.05). These data are the first to demonstrate that ARC is a specific, quantifiable and input dependent molecular marker of circuit activation which can serve to quantify the impact of brain injury and subsequent rehabilitation on whisker sensation.
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影响因子：
15.9
作者：
Korb E;Finkbeiner S
通讯作者：
Finkbeiner S
影响因子：
3.3
作者：
Ivanova, T. N.;Matthews, A.;Gross, C.;Mappus, R. C.;Gollnick, C.;Swanson, A.;Bassell, G. J.;Liu, R. C.
通讯作者：
Liu, R. C.
影响因子：
16.2
作者：
Plath, Niels;Ohana, Ora;Kuhl, Dietmar
通讯作者：
Kuhl, Dietmar
影响因子：
2.7
作者：
Chu, Yu-Fei;Yen, Chen-Tung;Lee, Li-Jen
通讯作者：
Lee, Li-Jen
影响因子：
3.5
作者：
Li, Lin;Yun, Sung Hwan;Tourtellotte, Warren G.
通讯作者：
Tourtellotte, Warren G.