New microbiological insights from the Bowland shale highlight heterogeneity of the hydraulically fractured shale microbiome.
New microbiological insights from the Bowland shale highlight heterogeneity of the hydraulically fractured shale microbiome.
复制标题
来自Bowland页岩的新的微生物学见解强调了水力压裂页岩微生物组的非均质性。
DOI：
10.1186/s40793-023-00465-1
复制
发表时间：
2023-02-28
影响因子：
7.9
通讯作者：
中科院分区：
文献类型：
--
作者：
水力压裂的页岩提供了一扇了解深层生物圈的窗口，在那里水力压裂在地球表面下几公里处创造了新的微生物生态系统。通过研究假定生活在这些环境中的回流液中的微生物群落，可以深入了解它们的生态生理学，特别是它们在这些极端环境中的生存能力，以及它们对现场操作的影响，例如通过有问题的生物污垢过程和/或生物腐蚀。在过去的十年里，对破裂页岩微生物的研究主要集中在北美的油井上，另有报道称，中国进行了几项研究。为了扩展这一领域的知识，我们对英国鲍兰页岩中两口勘探页岩气井的地球化学和微生物生态进行了表征。然后，我们使用荟萃分析方法，将我们研究地点的地球化学和16SRRNA基因测序数据与之前发表的横跨中国、加拿大和美国的不同地层的研究结果进行了比较。我们的发现表明，从鲍兰德地区的探井中回收的流体具有中等盐度和高微生物多样性的特点。微生物群落以已知能降解碳氢化合物的谱系为主，包括希瓦纳科、滨海杆菌科、盐藻科和假单胞菌科的成员。此外，英国破碎的页岩群落缺乏通常占主导地位的HalanAerobium谱系。从我们的荟萃分析中，我们推断氯离子浓度在控制微生物群落组成方面起主导作用。时空趋势也很明显，不同的页岩建造产生了具有不同多样性和组成的群落。这些发现突显出裂隙页岩地层的成分异质性达到了意想不到的水平，这不仅与管理实践相关，还提供了对不同微生物群体耐受工程深层地下极端条件的能力的洞察。网上版载有补充材料，可在10.1186/s40793-023-00465-1查阅。
Hydraulically fractured shales offer a window into the deep biosphere, where hydraulic fracturing creates new microbial ecosystems kilometers beneath the surface of the Earth. Studying the microbial communities from flowback fluids that are assumed to inhabit these environments provides insights into their ecophysiology, and in particular their ability to survive in these extreme environments as well as their influence on site operation e.g. via problematic biofouling processes and/or biocorrosion. Over the past decade, research on fractured shale microbiology has focused on wells in North America, with a few additional reported studies conducted in China. To extend the knowledge in this area, we characterized the geochemistry and microbial ecology of two exploratory shale gas wells in the Bowland Shale, UK. We then employed a meta-analysis approach to compare geochemical and 16S rRNA gene sequencing data from our study site with previously published research from geographically distinct formations spanning China, Canada and the USA. Our findings revealed that fluids recovered from exploratory wells in the Bowland are characterized by moderate salinity and high microbial diversity. The microbial community was dominated by lineages known to degrade hydrocarbons, including members of Shewanellaceae, Marinobacteraceae, Halomonadaceae and Pseudomonadaceae. Moreover, UK fractured shale communities lacked the usually dominant Halanaerobium lineages. From our meta-analysis, we infer that chloride concentrations play a dominant role in controlling microbial community composition. Spatio-temporal trends were also apparent, with different shale formations giving rise to communities of distinct diversity and composition. These findings highlight an unexpected level of compositional heterogeneity across fractured shale formations, which is not only relevant to inform management practices but also provides insight into the ability of diverse microbial consortia to tolerate the extreme conditions characteristic of the engineered deep subsurface. The online version contains supplementary material available at 10.1186/s40793-023-00465-1.
登录
查看更多内容
两种原油生产管道的代谢组和宏基因组分析，经历了腐蚀差异的差异。
影响因子：
5.2
作者：
Bonifay V;Wawrik B;Sunner J;Snodgrass EC;Aydin E;Duncan KE;Callaghan AV;Oldham A;Liengen T;Beech I
通讯作者：
Beech I
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
28.3
作者：
Daly, Rebecca A.;Borton, Mikayla A.;Wrighton, Kelly C.
通讯作者：
Wrighton, Kelly C.
影响因子：
3.6
作者：
Davis, James P.;Struchtemeyer, Christopher G.;Elshahed, Mostafa S.
通讯作者：
Elshahed, Mostafa S.
影响因子：
3.4
作者：
Akob, Denise M.;Cozzarelli, Isabelle M.;Lorah, Michelle M.
通讯作者：
Lorah, Michelle M.