Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers.
Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers.
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DOI：
10.3389/fpls.2015.00272
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发表时间：
2015
影响因子：
5.6
通讯作者：
Gong ZH
中科院分区：
文献类型：
--
作者：
Ji JJ;Huang W;Li Z;Chai WG;Yin YX;Li DW;Gong ZH
虽然辣椒细胞质雄性不育（CMS）与orf 507基因有关，但仍然缺乏直接导致雄性不育的确切证据。辣椒细胞质雄性不育系和保持系花药细胞色素c氧化酶的组织化学定位存在差异，主要分布在绒毡层细胞和绒毡层膜上。orf 507基因在辣椒保持系中的诱导和特异性表达发现，转化体在形态上与未转化和转化的对照植株相似，但具有皱缩的花药，表现出很少的开裂和较少的花粉粒，具有较低的发芽率和较高的自然损伤率。这些性状与不产生花粉的不育系不同。同时，以转化子为父本进行授粉试验，结果很少，且在有限数量的果实中有少量的种子。在四分体时期，转化体中发生了由程序性细胞死亡（PCD）引起的绒毡层细胞的消融，并且小孢子在单核时期变形和降解。通过测交证实了诱导半雄性不育的稳定传递。此外，orf 507在保持系中的表达在一定程度上抑制了atp 6 -2的表达，并导致细胞色素c氧化酶活性和线粒体F1 Fo-ATP合酶ATP水解能力的提高。这些结果说明了orf 507基因在绒毡层细胞中引起的早熟PCD和半雄性不育，而不是完全雄性不育。
Though cytoplasmic male sterility (CMS) in peppers is associated with the orf507 gene, definitive and direct evidence that it directly causes male sterility is still lacking. In this study, differences in histochemical localization of anther cytochrome c oxidase between the pepper CMS line and maintainer line were observed mainly in the tapetal cells and tapetal membrane. Inducible and specific expression of the orf507 gene in the pepper maintainer line found that transformants were morphologically similar to untransformed and transformed control plants, but had shrunken anthers that showed little dehiscence and fewer pollen grains with lower germination rate and higher naturally damaged rate. These characters were different from those of CMS line which does not produce any pollen grains. Meanwhile a pollination test using transformants as the male parent set few fruit and there were few seeds in the limited number of fruits. At the tetrad stage, ablation of the tapetal cell induced by premature programmed cell death (PCD) occurred in the transformants and the microspores were distorted and degraded at the mononuclear stage. Stable transmission of induced semi-male sterility was confirmed by a test cross. In addition, expression of orf507 in the maintainer lines seemed to inhibit expression of atp6-2 to a certain extent, and lead to the increase of the activity of cytochrome c oxidase and the ATP hydrolysis of the mitochondrial F1Fo-ATP synthase. These results introduce the premature PCD caused by orf507 gene in tapetal cells and semi-male sterility, but not complete male sterility.
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影响因子：
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作者：
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发表时间：
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影响因子：
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