An Alternative Culture Medium for Continuous In Vitro Propagation of the Human Pathogen Babesia duncani in Human Erythrocytes.
An Alternative Culture Medium for Continuous In Vitro Propagation of the Human Pathogen Babesia duncani in Human Erythrocytes.
复制标题
DOI：
10.3390/pathogens11050599
复制
发表时间：
2022-05-20
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
体外在人红细胞中持续繁殖的邓肯巴贝斯虫和小鼠致死性感染模型的建立使该寄生虫成为研究巴贝斯虫生物学和发病机制的理想模型。两种培养基，HL-1和Claycomb，具有专有配方，是已知唯一支持寄生虫在人红细胞中生长的培养基；然而，HL-1培养基已经停止使用，Claycomb培养基经常不可用，导致该病原体的研究严重中断。为了确定替代培养基条件，我们评估了邓肯双歧杆菌在各种成分明确的培养基中的生长情况。我们报告说，DMEM-F12培养基支持寄生虫在人红细胞中的持续生长，其水平与HL-1和Claycomb培养基中的水平相同。在此培养基中，我们从亲本WA-1临床分离物中连续三次亚克隆事件后产生了新的邓肯双球菌克隆。所有克隆的体外增殖率与WA-1亲本分离株相似，可致C3H/HeJ小鼠死亡。培养基可以很容易地从其单个成分中重组，以及这里开发的工具和资源将有助于对duncani的研究。
Continuous propagation of Babesia duncani in vitro in human erythrocytes and the availability of a mouse model of B. duncani lethal infection make this parasite an ideal model to study Babesia biology and pathogenesis. Two culture media, HL-1 and Claycomb, with proprietary formulations are the only culture media known to support the parasite growth in human erythrocytes; however, the HL-1 medium has been discontinued and the Claycomb medium is often unavailable leading to major interruptions in the study of this pathogen. To identify alternative media conditions, we evaluated the growth of B. duncani in various culture media with well-defined compositions. We report that the DMEM-F12 culture medium supports the continuous growth of the parasite in human erythrocytes to levels equal to those achieved in the HL-1 and Claycomb media. We generated new clones of B. duncani from the parental WA-1 clinical isolate after three consecutive subcloning events in this medium. All clones showed a multiplication rate in vitro similar to that of the WA-1 parental isolate and cause fatal infection in C3H/HeJ mice. The culture medium, which can be readily reconstituted from its individual components, and the tools and resources developed here will facilitate the study of B. duncani.
登录
查看更多内容
影响因子：
9.4
作者：
Limoncu, ME;Balcioglu, IC;Ozbilgin, A
通讯作者：
Ozbilgin, A
影响因子：
4.6
作者：
Silva JC;Cornillot E;McCracken C;Usmani-Brown S;Dwivedi A;Ifeonu OO;Crabtree J;Gotia HT;Virji AZ;Reynes C;Colinge J;Kumar V;Lawres L;Pazzi JE;Pablo JV;Hung C;Brancato J;Kumari P;Orvis J;Tretina K;Chibucos M;Ott S;Sadzewicz L;Sengamalay N;Shetty AC;Su Q;Tallon L;Fraser CM;Frutos R;Molina DM;Krause PJ;Ben Mamoun C
通讯作者：
Ben Mamoun C
影响因子：
4.8
作者：
Downie, Megan J.;El Bissati, Kamal;Mamoun, Choukri Ben
通讯作者：
Mamoun, Choukri Ben
影响因子：
2.9
作者：
Kjemtrup, AM;Lee, B;Conrad, PA
通讯作者：
Conrad, PA
影响因子：
39.2
作者：
Herwaldt, BL;Persing, DH;Gorenflot, AF
通讯作者：
Gorenflot, AF