Structure and properties of transcriptional networks driving selenite stress response in yeasts.
Structure and properties of transcriptional networks driving selenite stress response in yeasts.
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DOI：
10.1186/1471-2164-9-333
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发表时间：
2008-07-15
期刊：
影响因子：
4.4
通讯作者：
Devaux F
中科院分区：
文献类型：
--
作者：
Salin H;Fardeau V;Piccini E;Lelandais G;Tanty V;Lemoine S;Jacq C;Devaux F
压力响应提供了有价值的模型，用于破译控制细胞对环境的适应的转录网络。我们分析了酵母对亚硒酸盐毒性浓度的转录组反应。我们使用基因网络映射工具来识别此响应中涉及的功能途径和转录因子。然后，我们使用染色质免疫沉淀和敲除实验来研究其中一些调节剂的作用以及它们之间的调节连接。
亚硒酸盐迅速激活一系列转录回路，包括铁剥夺，氧化应激和蛋白质降解反应。几个转录调节剂的mRNA水平本身受到调节。我们证明了将蛋白酶体表达的调节剂RPN4P与多效性药物反应因子PDR1P连接的阳性转录环的存在。我们还提供了该调节模块参与由YAP1P转录因子控制的氧化应激反应及其在致病性酵母菌glabrata中的氧化应激反应的证据。此外，我们表明耐药性调节剂基因YRR1和铁稳态调节基因AFT2均受YAP1P的直接调节。
这项工作描述了一个高度相互联系且复杂的转录网络，涉及酵母基因组表达在其化学环境中存在于硒岩的存在。它揭示了RPN4P对PDR1的转录调控，它提出了多效毒素耐药性网络在压力反应中的新作用，并证明了氧化应激反应与铁稳态之间的直接调节联系。
Stress responses provide valuable models for deciphering the transcriptional networks controlling the adaptation of the cell to its environment. We analyzed the transcriptome response of yeast to toxic concentrations of selenite. We used gene network mapping tools to identify functional pathways and transcription factors involved in this response. We then used chromatin immunoprecipitation and knock-out experiments to investigate the role of some of these regulators and the regulatory connections between them. Selenite rapidly activates a battery of transcriptional circuits, including iron deprivation, oxidative stress and protein degradation responses. The mRNA levels of several transcriptional regulators are themselves regulated. We demonstrate the existence of a positive transcriptional loop connecting the regulator of proteasome expression, Rpn4p, to the pleiotropic drug response factor, Pdr1p. We also provide evidence for the involvement of this regulatory module in the oxidative stress response controlled by the Yap1p transcription factor and its conservation in the pathogenic yeast C. glabrata. In addition, we show that the drug resistance regulator gene YRR1 and the iron homeostasis regulator gene AFT2 are both directly regulated by Yap1p. This work depicted a highly interconnected and complex transcriptional network involved in the adaptation of yeast genome expression to the presence of selenite in its chemical environment. It revealed the transcriptional regulation of PDR1 by Rpn4p, proposed a new role for the pleiotropic drug resistance network in stress response and demonstrated a direct regulatory connection between oxidative stress response and iron homeostasis.
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影响因子：
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