Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae.
Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae.
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DOI：
10.1186/gb-2005-6-9-r77
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发表时间：
2005
期刊：
影响因子：
12.3
通讯作者：
Harper JF
中科院分区：
文献类型：
--
作者：
Eide DJ;Clark S;Nair TM;Gehl M;Gribskov M;Guerinot ML;Harper JF
在4，385株酵母突变株中测定了13种矿物质的积累，鉴定出212株显示出改变的离子组（矿物质积累）谱。营养矿物质是必不可少的，但潜在的毒性，和稳态机制需要调节其细胞内水平。在这里，我们描述了一个全基因组的筛选基因参与的矿物质在酿酒酵母的稳态。使用电感耦合等离子体原子发射光谱法（ICP-AES），我们测定了4，385株突变菌株中13种元素（钙、钴、铜、铁、钾、镁、锰、镍、磷、硒、钠、硫和锌）的积累。我们将所得的积累概况称为酵母“离子组”。我们鉴定了212株在丰富的生长培养基上生长时显示出改变的离子组谱。令人惊讶的是，这些突变体（4株）中很少有只受一种元素的影响。相反，在大多数突变体中，多种元素的水平发生了改变。同样值得注意的是，只有六个基因先前被证明参与矿物质的吸收和利用，这表明在这些充足的条件下，体内平衡是稳健的。许多突变体确定影响线粒体或液泡功能，这些群体表现出类似的影响，许多不同的元素的积累。此外，还观察到不同元素之间有趣的正相关和负相关。最后，离子组图谱数据使我们能够正确预测以前未表征的基因YDR 065 W的功能。我们表明，该基因是必需的液泡酸化。我们的研究结果表明，离子组学的力量，以确定新的方面的矿物质稳态和这些数据如何可以用来发展假说的功能，以前未表征的基因。
The accumulation of thirteen minerals was assayed in 4,385 yeast mutant strains, identifying 212 strains that showed altered ionome (mineral accumulation) profiles. Nutrient minerals are essential yet potentially toxic, and homeostatic mechanisms are required to regulate their intracellular levels. We describe here a genome-wide screen for genes involved in the homeostasis of minerals in Saccharomyces cerevisiae. Using inductively coupled plasma-atomic emission spectroscopy (ICP-AES), we assayed 4,385 mutant strains for the accumulation of 13 elements (calcium, cobalt, copper, iron, potassium, magnesium, manganese, nickel, phosphorus, selenium, sodium, sulfur, and zinc). We refer to the resulting accumulation profile as the yeast 'ionome'. We identified 212 strains that showed altered ionome profiles when grown on a rich growth medium. Surprisingly few of these mutants (four strains) were affected for only one element. Rather, levels of multiple elements were altered in most mutants. It was also remarkable that only six genes previously shown to be involved in the uptake and utilization of minerals were identified here, indicating that homeostasis is robust under these replete conditions. Many mutants identified affected either mitochondrial or vacuolar function and these groups showed similar effects on the accumulation of many different elements. In addition, intriguing positive and negative correlations among different elements were observed. Finally, ionome profile data allowed us to correctly predict a function for a previously uncharacterized gene, YDR065W. We show that this gene is required for vacuolar acidification. Our results indicate the power of ionomics to identify new aspects of mineral homeostasis and how these data can be used to develop hypotheses regarding the functions of previously uncharacterized genes.
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DOI：
10.1083/jcb.200203052
发表时间：
2002-06-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cronin SR;Rao R;Hampton RY
通讯作者：
Hampton RY
影响因子：
11.4
作者：
Ooi, CE;Rabinovich, E;Klausner, RD
通讯作者：
Klausner, RD
影响因子：
4.8
作者：
Li, LT;Chen, OS;Kaplan, J
通讯作者：
Kaplan, J
影响因子：
4.8
作者：
Chan, SY;Appling, DR
通讯作者：
Appling, DR
影响因子：
3.3
作者：
Ogawa, N;DeRisi, J;Brown, PO
通讯作者：
Brown, PO