Bladder and bowel responses to lumbosacral epidural stimulation in uninjured and transected anesthetized rats.
Bladder and bowel responses to lumbosacral epidural stimulation in uninjured and transected anesthetized rats.
复制标题
DOI：
10.1038/s41598-021-81822-3
复制
发表时间：
2021-02-08
影响因子：
4.6
通讯作者：
Hubscher CH
中科院分区：
文献类型：
--
作者：
Hoey RF;Medina-Aguiñaga D;Khalifa F;Ugiliweneza B;Zdunowski S;Fell J;Naglah A;El-Baz AS;Herrity AN;Harkema SJ;Hubscher CH
在L5-S1进行脊髓硬膜外刺激（scES）标测，以确定膀胱和肠抑制和/或收缩的参数。使用脊柱完整和慢性横断大鼠的两种性别的急性脊髓麻醉的终端准备，scES系统地应用在膀胱充盈/排空周期，同时记录膀胱和结肠直肠的压力和外部尿道和肛门括约肌肌电图活动的修改Specify 5-6-5（美敦力）电极。结果表明，排尿量，排尿，肠蠕动和括约肌活动的频率依赖性影响，高于可视化的运动阈值强度不同，取决于神经系统的完整性，与一些性别依赖性差异。此后，测试了为更大选择性而设计的定制设计的微型15电极阵列，并在小得多的表面积上表现出相同的频率依赖性泌尿效应，而没有任何并发运动。因此，选择激活自主神经系统电路与scES是一个有前途的神经调节方法，方便翻译为个人与SCI和潜在的其他神经系统疾病。
Spinal cord epidural stimulation (scES) mapping at L5-S1 was performed to identify parameters for bladder and bowel inhibition and/or contraction. Using spinally intact and chronic transected rats of both sexes in acute urethane-anesthetized terminal preparations, scES was systematically applied using a modified Specify 5–6–5 (Medtronic) electrode during bladder filling/emptying cycles while recording bladder and colorectal pressures and external urethral and anal sphincter electromyography activity. The results indicate frequency-dependent effects on void volume, micturition, bowel peristalsis, and sphincter activity just above visualized movement threshold intensities that differed depending upon neurological intactness, with some sex-dependent differences. Thereafter, a custom-designed miniature 15-electrode array designed for greater selectivity was tested and exhibited the same frequency-dependent urinary effects over a much smaller surface area without any concurrent movements. Thus, select activation of autonomic nervous system circuitries with scES is a promising neuromodulation approach for expedient translation to individuals with SCI and potentially other neurologic disorders.
登录
查看更多内容
影响因子：
--
作者：
Choudhary, Mahipal;van Mastrigt, Ron;van Asselt, Els
通讯作者：
van Asselt, Els
影响因子：
3.6
作者：
Bernabe, Jacques;Clement, Pierre;Giuliano, Francois
通讯作者：
Giuliano, Francois
影响因子：
2.6
作者：
Choudhary, Mahipal;van Mastrigt, Ron;van Asselt, Els
通讯作者：
van Asselt, Els
影响因子：
2
作者：
de Groat, William C.;Yoshimura, Naoki
通讯作者：
Yoshimura, Naoki
影响因子：
2
作者：
Fraser MO;Smith PP;Sullivan MP;Bjorling DE;Campeau L;Andersson KE;Yoshiyama M
通讯作者：
Yoshiyama M