Mesenchymal Stem Cells Facilitate In Vitro Development of Human Preantral Follicle
Mesenchymal Stem Cells Facilitate In Vitro Development of Human Preantral Follicle
复制标题
间充质干细胞促进人类腔前卵泡的体外发育
DOI：
10.1177/1933719115578922
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发表时间：
2015-04
影响因子：
2.9
通讯作者：
Qiao Jie
中科院分区：
文献类型：
--
作者：
Xia Xi;Wang Tianren;Yin Tailang;Yan Liying;Yan Jie;Lu Cuilin;Zhao Liang;Li Min;Zhang Yan;Jin Hongyan;Zhu Xiaohui;Liu Ping;Li Rong;Qiao Jie
生物学卵泡发生是一个漫长而复杂的过程，卵泡生长的微环境知之甚少。间充质干细胞（MSC）已被证明可以为伤口修复，自身免疫性疾病改善和肿瘤发展建立支持性微环境。因此，本研究旨在探讨间充质干细胞是否可以帮助重建微环境，以促进体外卵泡发育。在这里，我们显示人MSC显著提高存活率，增加生长速度，并以剂量依赖的方式提高腔前卵泡的活力。进一步分析发现，与MSCs共培养的卵泡中卵母细胞中的生长分化因子9和骨形态发生蛋白15以及颗粒细胞中的β-淀粉样蛋白βA和转化生长因子β1的表达均显著高于无MSCs培养的卵泡。总之，我们的研究结果证明了以前未被认识到的功能，骨髓间充质干细胞在促进腔前卵泡发育，并提供了一个有用的策略，以优化生育力的保存和恢复，促进体外卵泡生长。
Biological folliculogenesis is a lengthy and complicated process, and follicle growth microenvironment is poorly understood. Mesenchymal stem cells (MSCs) have been shown to establish a supportive microenvironment for wound repair, autoimmune diseases amelioration, and tumor development. Therefore, this study is aimed to investigate whether MSCs could help to reconstruct a microenvironment to facilitate the in vitro follicle development. Here we show human MSCs significantly promote the survival rates, increase the growth velocity, and improve the viability of preantral follicles in a dose-dependent manner. Further analyses reveal that growth differentiation factor 9 and bone morphogenetic protein 15 in oocytes and inhibin βA and transforming growth factor β1 in granulose cells within the follicles cocultured with MSCs express notably higher than those in the follicles cultured without MSCs. In summary, our findings demonstrate a previously unrecognized function of MSCs in promoting preantral follicle development and provide a useful strategy to optimize fertility preservation and restoration by facilitating in vitro follicle growth.
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影响因子：
82.9
作者：
通讯作者：
--
影响因子：
3.7
作者：
Roubelakis MG;Tsaknakis G;Pappa KI;Anagnou NP;Watt SM
通讯作者：
Watt SM
影响因子：
23.9
作者：
Caplan AI;Correa D
通讯作者：
Correa D
DOI：
10.1095/biolreprod65.3.967
发表时间：
2001-09
期刊：
--
影响因子：
--
作者：
Jia Zhao;M. Taverne;G. C. van der Weijden;M. Bevers;R. van den Hurk
通讯作者：
Jia Zhao;M. Taverne;G. C. van der Weijden;M. Bevers;R. van den Hurk
影响因子：
6.1
作者：
Jing Xu;M. Lawson;R. Yeoman;K. Pau;S. Barrett;M. Zelinski;R. Stouffer;R. Stouffer
通讯作者：
Jing Xu;M. Lawson;R. Yeoman;K. Pau;S. Barrett;M. Zelinski;R. Stouffer;R. Stouffer