Studies of new EST-SSRs derived from Gossypium barbadense
Studies of new EST-SSRs derived from Gossypium barbadense
复制标题
海岛棉新 EST-SSR 的研究
DOI：
10.1007/s11434-007-0399-2
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发表时间：
2007-09
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
现有的棉花EST-SSR标记主要来源于木棉（Gossypium arboreum）和毛棉（Gossypium hirsutum），而来自棉的EST-SSR标记较少。利用巴氏酵母的发育纤维构建cDNA文库，利用其中的98条独特ESTs，构建了191条EST-SSRs。pima3 - 79。在SSRs中，三核苷酸AAG出现频率最高，为11.76%。利用36份材料（包括A基因组的13个二倍体、D基因组的11个二倍体和AD基因组的12个异源四倍体）检测新的EST-SSRs。共扩增76个EST-SSRs，获得313个多态性片段，平均每对引物有4.11个多态性片段。PIC范围为0.17 ~ 0.95，平均值为0.53。根据Jaccard遗传相似系数，将36份材料聚为3组。21个EST-SSRs在BC1群体（（Emian22 × Pima3-79） × Emian22）中表现出多态性，产生了24个多态性位点，其中22个多态性位点与我们的BC1种间主干遗传连锁图谱整合，锚定在12条染色体上。
Existing cotton EST-SSR markers are mostly derived from Gossypium arboreum and Gossypium hirsutum, but EST-SSR markers from Gossypium barbadense are scarce. One hundred and nineteen EST-SSRs were developed based on 98 unique ESTs from a cDNA library constructed in our laboratory using developing fibers from G. barbadense cv. Pima3–79. Among the SSRs, trinucleotide AAG appeared at a high frequency of 11.76%. 36 accessions (consisting of 13 diploids of the A genome, 11 diploids of the D genome and 12 allotetraploids of the AD genome) were employed to test new EST-SSRs. 76 EST-SSRs were successfully amplified, and 313 polymorphic fragments were yielded, with an average of 4.11 fragments per primer pair. The PIC ranged from 0.17 to 0.95 with an average of 0.53. Based on Jaccard’s genetic similarity coefficient, these 36 accessions were clustered into three groups. 21 EST-SSRs exhibited polymorphisms in BC1 population ((Emian22 × Pima3–79) × Emian22), 24 polymorphic loci were generated, while 22 of the 24 polymorphic loci were integrated with our interspecific BC1 backbone genetic linkage map, and anchored in 12 chromosomes.
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影响因子：
2.1
作者：
Paterson, A. H.;Brubaker, C. L.;Wendel, J. F.
通讯作者：
Wendel, J. F.
影响因子：
5.4
作者：
Scott, KD;Eggler, P;Henry, RJ
通讯作者：
Henry, RJ
影响因子：
9.8
作者：
J. Weber;P. May
通讯作者：
J. Weber;P. May
DOI：
--
发表时间：
1958-01
期刊：
University of Kansas science bulletin
影响因子：
--
作者：
R. Sokal;C. Michener
通讯作者：
R. Sokal;C. Michener
影响因子：
1
作者：
P. Gupta;H. Balyan;P. C. Sharma
通讯作者：
P. Gupta;H. Balyan;P. C. Sharma