The Hypothalamo‐Pituitary‐Adrenal Axis of Athymic Swiss Nude Mice: The Implications of T Lymphocytes in the ACTH Release from Immune Cells a
The Hypothalamo‐Pituitary‐Adrenal Axis of Athymic Swiss Nude Mice: The Implications of T Lymphocytes in the ACTH Release from Immune Cells a
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无胸腺瑞士裸鼠的下丘脑-垂体-肾上腺轴：T 淋巴细胞对免疫细胞释放 ACTH 的影响
DOI：
10.1111/j.1749-6632.1998.tb09586.x
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发表时间：
1998
影响因子：
5.2
通讯作者：
E. Spinedi
中科院分区：
文献类型：
--
作者：
R. Gaillard;T. Daneva;R. Hadid;K. Muller;E. Spinedi
摘要：由于胸腺在免疫功能调节中的作用众所周知，我们研究了胸腺的缺乏是否会影响下丘脑-垂体-肾上腺(HPA)轴的活动。用8周龄的雌性瑞士裸鼠和BALB/c小鼠分别研究了(A)HPA轴对下丘脑、垂体或肾上腺水平的各种应激和刺激的“在体”反应，以及(B)垂体和肾上腺细胞对CRH和ACTH刺激的体外反应。结果表明：(1)瑞士裸鼠基础血浆ACTH水平显著高于BALB/c小鼠(p<；0.05)，而基础皮质酮(B)水平与BALB/c小鼠相似；(2)无论施加何种刺激，瑞士裸鼠血浆中ACTH和B的释放均显著低于BALB/c小鼠(p<；0.05)；(3)对CRH的“体外”垂体反应和对ACTH的肾上腺反应显著低于BALB/c小鼠(p<；0.05)；(4)两品系小鼠下丘脑CRH和垂体ACTH含量相近，而无瘤小鼠肾上腺B含量显著降低(P<0.05)。通过注射同基因杂合(即免疫完全合格)供者的脾细胞对裸鼠进行免疫重建，裸鼠的B肾上腺含量显著增加。在脾细胞中，CD4+T淋巴细胞在免疫细胞释放ACTH中起着特别重要的作用。总而言之，我们的结果表明，裸鼠对各种压力和刺激都有迟钝的HPA轴反应；这一缺陷似乎存在于脑垂体和肾上腺水平。裸鼠的免疫重建导致肾上腺B含量正常化。
Abstract: Because of the well‐known role of the thymus in the regulation of immune function, we investigated whether the lack of thymus may affect hypothalamo‐pituitary‐adrenal (HPA) axis activity. Eight‐week‐old female Swiss nude (athymic) and BALB/c (normal) mice were used to study (a) the “in vivo” response of the HPA axis to various stresses and stimuli acting at either hypothalamic, pituitary, or adrenal levels and (b) the in vitro response of pituitary and adrenal cells to CRH and ACTH stimulation, respectively. The results indicate that (1) basal plasma ACTH levels were significantly (p < 0.05) higher in Swiss nude than in BALB/c mice, whereas basal corticosterone (B) levels were similar in both strains of mice; (2) the stress‐induced release of ACTH and B in plasma was significantly (p < 0.05) lower in Swiss nude than in BALB/c mice, regardless of the stimulus applied; (3) the “in vitro” pituitary response to CRH and the adrenal response to ACTH were significantly (p < 0.05) lower in Swiss nude than in BALB/c mice; and (4) whereas hypothalamic CRH and pituitary ACTH contents were similar in both strains, adrenal B content was significantly (p < 0.05) lower in athymic mice. Immune reconstitution of the athymic nude mice by injecting splenocytes obtained from syngeneic heterozygous (i.e., immunologically fully competent) donors produced a significant increase in the B adrenal content of the nude mice. Among the splenocytes, CD4+ T‐lymphocytes play a particularly important role in the release of ACTH from cells of the immune system. In conclusion, our results indicate that athymic nude mice have a blunted HPA axis response to various stresses and stimuli; this defect seems to reside at both the pituitary and adrenal levels. Immune reconstitution of the nude mice leads to a normalization of the adrenal B content.
影响因子：
56.9
作者：
R. Rebar;A. Miyake;T. Low;A. Goldstein
通讯作者：
R. Rebar;A. Miyake;T. Low;A. Goldstein
胸腺肽 5 (TF5) 刺激培养的大鼠垂体分泌促肾上腺皮质激素 (ACTH)。
影响因子：
6.1
作者：
McGillis,JP;Hall,NR;Goldstein,AL
通讯作者：
Goldstein,AL
胸腺-肾上腺连接：胸腺素在灵长类动物中具有促肾上腺皮质激素释放活性。
DOI：
10.1126/science.6318312
发表时间：
1983
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Healy,DL;Hodgen,GD;Schulte,HM;Chrousos,GP;Loriaux,DL;Hall,NR;Goldstein,AL
通讯作者：
Goldstein,AL