The Candida albicans HIR histone chaperone regulates the yeast-to-hyphae transition by controlling the sensitivity to morphogenesis signals.
The Candida albicans HIR histone chaperone regulates the yeast-to-hyphae transition by controlling the sensitivity to morphogenesis signals.
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DOI：
10.1038/s41598-017-08239-9
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发表时间：
2017-08-16
影响因子：
4.6
通讯作者：
Kuchler K
中科院分区：
文献类型：
--
作者：
Jenull S;Tscherner M;Gulati M;Nobile CJ;Chauhan N;Kuchler K
形态可塑性，如酵母到菌丝的转变是人类真菌病原体白色念珠菌的关键毒力因子。菌丝的形成是由一个由环境感知、信号传导、转录调节因子以及染色质修饰组成的多层调控网络控制的。在这里，我们证明了一个新的作用，复制独立的HIR组蛋白伴侣复合物在真菌形态发生。HIR作为菌丝发育的重要调节剂，因为HIR复合物亚基Hir 1的遗传消融降低了对形态发生刺激的敏感性。引人注目的是，HIR 1缺陷细胞显示改变的转录幅度菌丝启动后，表明Hir 1影响转录，通过建立转录阈值所需的驱动形态发生细胞命运的决定。此外，促进菌丝维持的转录因子Ume 6的异位表达挽救了hir 1 Δ/Δ细胞的表达缺陷，表明Hir 1影响菌丝起始的早期阶段。因此，染色质伴侣介导的转录微调对于驱动真菌病原体C中的形态发生转化至关重要。白色念珠菌
Morphological plasticity such as the yeast-to-hyphae transition is a key virulence factor of the human fungal pathogen Candida albicans. Hyphal formation is controlled by a multilayer regulatory network composed of environmental sensing, signaling, transcriptional modulators as well as chromatin modifications. Here, we demonstrate a novel role for the replication-independent HIR histone chaperone complex in fungal morphogenesis. HIR operates as a crucial modulator of hyphal development, since genetic ablation of the HIR complex subunit Hir1 decreases sensitivity to morphogenetic stimuli. Strikingly, HIR1-deficient cells display altered transcriptional amplitudes upon hyphal initiation, suggesting that Hir1 affects transcription by establishing transcriptional thresholds required for driving morphogenetic cell-fate decisions. Furthermore, ectopic expression of the transcription factor Ume6, which facilitates hyphal maintenance, rescues filamentation defects of hir1Δ/Δ cells, suggesting that Hir1 impacts the early phase of hyphal initiation. Hence, chromatin chaperone-mediated fine-tuning of transcription is crucial for driving morphogenetic conversions in the fungal pathogen C. albicans.
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影响因子：
64.5
作者：
Banaszynski LA;Wen D;Dewell S;Whitcomb SJ;Lin M;Diaz N;Elsässer SJ;Chapgier A;Goldberg AD;Canaani E;Rafii S;Zheng D;Allis CD
通讯作者：
Allis CD
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
3.4
作者：
Barelle CJ;Priest CL;Maccallum DM;Gow NA;Odds FC;Brown AJ
通讯作者：
Brown AJ
影响因子：
2.9
作者：
BLUM, H;BEIER, H;GROSS, HJ
通讯作者：
GROSS, HJ
影响因子：
16.8
作者：
Burgess, Rebecca J.;Zhang, Zhiguo
通讯作者：
Zhang, Zhiguo