Additively manufactured biodegradable porous magnesium implants for elimination of implant-related infections: An in vitro and in vivo study.
Additively manufactured biodegradable porous magnesium implants for elimination of implant-related infections: An in vitro and in vivo study.
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用于消除植入物相关感染的增材制造的可生物降解多孔镁植入物：一项体外和体内研究
DOI：
10.1016/j.bioactmat.2021.06.032
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发表时间：
2022-03
影响因子：
18.9
通讯作者：
Hao Y
中科院分区：
文献类型：
--
作者：
Xie K;Wang N;Guo Y;Zhao S;Tan J;Wang L;Li G;Wu J;Yang Y;Xu W;Chen J;Jiang W;Fu P;Hao Y
镁（Mg）合金具有抗菌和成骨的特性，是骨科植入物的合适人选。然而，制造适合骨修复的理想镁植入物仍然具有挑战性，因为它要求植入物具有相互连接的孔结构和个性化的几何形状。在本研究中，我们使用选择性激光熔化技术制备了具有合适力学性能的多孔3d打印Mg-Nd-Zn-Zr（标记为JDBM）植入物。3d打印的JDBM种植体在MC3T3-E1和RAW267.4细胞中具有细胞相容性，体外具有良好的骨诱导能力。此外，该种植体对耐甲氧西林金黄色葡萄球菌（MRSA）和大肠杆菌的抑菌率分别为90.0%和92.1%。3d打印的JDBM植入物在兔模型中可防止mrsa诱导的植入物相关感染，并且根据组织学评估、血液检查和Mg2+沉积检测结果显示出良好的体内生物相容性。此外，3d打印的JDBM种植体在种植早期观察到骨-种植界面的炎症反应和TNF-α分泌增强。3d打印JDBM植入物降解产生的高Mg2+环境可促进巨噬细胞M1表型（Tnf、iNOS、Ccl3、Ccl4、Ccl5、Cxcl10、Cxcl2），增强巨噬细胞的吞噬能力。mg基种植体在种植初期相对较快的Mg2+释放和种植体降解所产生的增强的免疫调节作用是其潜在的抗菌机制。我们的研究结果表明，3d打印多孔JDBM植入物具有抗菌性能和骨诱导性，在未来的骨科应用中具有潜力。采用选择性激光熔融法制备了具有良好力学性能的多孔JDBM植入物。3d打印的JDBM种植体具有良好的抗菌性能、骨诱导性和生物相容性。早期时间性增强的免疫调节作用可能是镁基种植体抗菌的潜在机制。
Magnesium (Mg) alloys that have both antibacterial and osteogenic properties are suitable candidates for orthopedic implants. However, the fabrication of ideal Mg implants suitable for bone repair remains challenging because it requires implants with interconnected pore structures and personalized geometric shapes. In this study, we fabricated a porous 3D-printed Mg-Nd-Zn-Zr (denoted as JDBM) implant with suitable mechanical properties using selective laser melting technology. The 3D-printed JDBM implant exhibited cytocompatibility in MC3T3-E1 and RAW267.4 cells and excellent osteoinductivity in vitro. Furthermore, the implant demonstrated excellent antibacterial ratios of 90.0% and 92.1% for methicillin-resistant S. aureus (MRSA) and Escherichia coli, respectively. The 3D-printed JDBM implant prevented MRSA-induced implant-related infection in a rabbit model and showed good in vivo biocompatibility based on the results of histological evaluation, blood tests, and Mg2+ deposition detection. In addition, enhanced inflammatory response and TNF-α secretion were observed at the bone-implant interface of the 3D-printed JDBM implants during the early implantation stage. The high Mg2+ environment produced by the degradation of 3D-printed JDBM implants could promote M1 phenotype of macrophages (Tnf, iNOS, Ccl3, Ccl4, Ccl5, Cxcl10, and Cxcl2), and enhance the phagocytic ability of macrophages. The enhanced immunoregulatory effect generated by relatively fast Mg2+ release and implant degradation during the early implantation stage is a potential antibacterial mechanism of Mg-based implant. Our findings indicate that 3D-printed porous JDBM implants, having both antibacterial property and osteoinductivity, hold potential for future orthopedic applications. Porous JDBM implants promising mechanical properties was fabricated by selective laser melting. 3D-printed JDBM implant exhibited excellent antibacterial property, osteoinductivity, and biocompatibility. Temporally enhanced immunoregulatory effect in early stage was a potential antibacterial mechanism of Mg-based implant.
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