Dynamics of transcriptome changes during subcutaneous preadipocyte differentiation in ducks
Dynamics of transcriptome changes during subcutaneous preadipocyte differentiation in ducks
复制标题
鸭皮下前脂肪细胞分化过程中转录组变化的动态
DOI：
10.1186/s12864-019-6055-9
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发表时间：
2019-09
期刊：
影响因子：
4.4
通讯作者：
Hou Zhuocheng
中科院分区：
文献类型：
--
作者：
Wang Zheng;Yin Zhong Tao;Zhang Fan;Li Xiao Qin;Chen Si Rui;Yang Ning;Porter Tom E;Hou Zhuocheng
背景北京鸭是一种重要的脂肪合成和沉积动物模型。然而，在这个模型中，由复杂的信号级联驱动的脂肪分化的转录动态调节在很大程度上仍未被探索。本研究旨在探讨成脂转录动力学前（增殖）和后（分化）初始前脂肪细胞分化duck.ResultsExogenous油酸单独成功诱导鸭皮下前脂肪细胞分化。我们探索了36个mRNA-seq文库，以研究增殖和分化过程中6个时间点的转录组动态。使用稳健的统计分析，我们分别鉴定了845、652、359、2401和1933个基因在-48 h和0 h、0 h和12 h、12 h和24 h、24 h和48 h、48 h和72 h之间差异表达（FDR < 0.05，FC > 1.5）。在增殖阶段，与增殖相关的途径以及基本的细胞和代谢过程被抑制，而启动分化的调节因子进入准备激活状态，这为启动脂肪分化提供了先决条件。根据加权基因共表达网络分析，与成脂分化正相关的通路在分化阶段被显著激活，而WNT、FOXO等抑制前脂肪细胞分化的通路被负调控。此外，我们确定和分类超过100个转录因子，在分化过程中表现出显着的变化，并发现新的转录因子，没有报道与preadipotye分化。最后，根据表达数据人工构建了鸭皮下前脂肪细胞分化的调控网络模型，并提出E2 F1可能是鸭皮下前脂肪细胞增殖和分化过程中的重要环节。本研究为了解鸭皮下脂肪的合成和沉积提供了坚实的基础。此外，所产生的信息将允许未来的调查参与特定阶段的鸭脂肪形成的特定基因。
BackgroundPekin duck is an important animal model for its ability for fat synthesis and deposition. However, transcriptional dynamic regulation of adipose differentiation driven by complex signal cascades remains largely unexplored in this model. This study aimed to explore adipogenic transcriptional dynamics before (proliferation) and after (differentiation) initial preadipocyte differentiation in ducks.ResultsExogenous oleic acid alone successfully induced duck subcutaneous preadipocyte differentiation. We explored 36 mRNA-seq libraries in order to study transcriptome dynamics during proliferation and differentiation processes at 6 time points. Using robust statistical analysis, we identified 845, 652, 359, 2401 and 1933 genes differentially expressed between -48 h and 0 h, 0 h and 12 h, 12 h and 24 h, 24 h and 48 h, 48 h and 72 h, respectively (FDR < 0.05, FC > 1.5). At the proliferation stage, proliferation related pathways and basic cellular and metabolic processes were inhibited, while regulatory factors that initiate differentiation enter the ready-to-activate state, which provides a precondition for initiating adipose differentiation. According to weighted gene co-expression network analysis, pathways positively related to adipogenic differentiation are significantly activated at the differentiation stage, while WNT, FOXO and other pathways that inhibit preadipocyte differentiation are negatively regulated. Moreover, we identified and classified more than 100 transcription factors that showed significant changes during differentiation, and found novel transcription factors that were not reported to be related to preadipoctye differentiation. Finally, we manually assembled a proposed regulation network model of subcutaneous preadipocyte differentiation base on the expression data, and suggested thatE2F1may serve as an important link between the processes of duck subcutaneous preadipocyte proliferation and differentiation.ConclusionsFor the first time we comprehensively analyzed the transcriptome dynamics of duck subcutaneous preadipocyte proliferation and differentiation. The current study provides a solid basis for understanding the synthesis and deposition of subcutaneous fat in ducks. Furthermore, the information generated will allow future investigations of specific genes involved in particular stages of duck adipogenesis.
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影响因子：
--
作者：
Ross, DA;Hannenhalli, S;Kadesch, T
通讯作者：
Kadesch, T
控制不同脂肪库中脂肪细胞分化；对病理生理或治疗的影响。
影响因子：
5.2
作者：
Ma X;Lee P;Chisholm DJ;James DE
通讯作者：
James DE
影响因子：
12.4
作者：
Chen Q;Shou P;Zheng C;Jiang M;Cao G;Yang Q;Cao J;Xie N;Velletri T;Zhang X;Xu C;Zhang L;Yang H;Hou J;Wang Y;Shi Y
通讯作者：
Shi Y
DOI：
--
发表时间：
1997
期刊：
The EMBO journal
影响因子：
--
作者：
T. Tanaka;N. Yoshida;T. Kishimoto;S. Akira
通讯作者：
T. Tanaka;N. Yoshida;T. Kishimoto;S. Akira
WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S