Curcumin Stimulates Proliferation of Spinal Cord Neural Progenitor Cells via a Mitogen-Activated Protein Kinase Signaling Pathway.
Curcumin Stimulates Proliferation of Spinal Cord Neural Progenitor Cells via a Mitogen-Activated Protein Kinase Signaling Pathway.
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DOI：
10.3340/jkns.2014.56.1.1
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发表时间：
2014-07
影响因子：
1.6
通讯作者：
Bae JS
中科院分区：
文献类型：
--
作者：
Son S;Kim KT;Cho DC;Kim HJ;Sung JK;Bae JS
本研究旨在探讨姜黄素对脊髓神经前体细胞(SC-NPC)增殖的影响，并阐明SC-NPC中丝裂原活化蛋白(MAP)信号转导通路的作用机制。我们从体重250~350g的SD大鼠脊髓提取的干细胞-神经干细胞建立了培养体系，并测定了不同剂量姜黄素对干细胞-神经干细胞的增殖率。免疫印迹法检测含有细胞外信号调节蛋白(ERKs)、p38、c-Jun NH2末端蛋白(JNKs)和β-肌动蛋白的MAP激酶信号蛋白。姜黄素对SC-NPC的增殖具有双向作用。较低剂量(0.1、0.5、1µM)的姜黄素可促进SC-NPC的增殖。但是，较高剂量可抑制SC-NPC的增殖。此外，姜黄素还通过MAP激酶信号通路，尤其是p-ERK和p-38蛋白，刺激SC-NC的增殖。P-ERK蛋白和p38蛋白水平随姜黄素剂量的不同而不同(0.5和1微米，p<；0.05)。姜黄素在低浓度时可通过ERKs和p38信号通路刺激SC-NC的增殖。
The aims of our study are to evaluate the effect of curcumin on spinal cord neural progenitor cell (SC-NPC) proliferation and to clarify the mechanisms of mitogen-activated protein (MAP) kinase signaling pathways in SC-NPCs. We established cultures of SC-NPCs, extracted from the spinal cord of Sprague-Dawley rats weighing 250 g to 350 g. We measured proliferation rates of SC-NPCs after curcumin treatment at different dosage. The immuno-blotting method was used to evaluate the MAP kinase signaling protein that contains extracellular signal-regulated kinases (ERKs), p38, c-Jun NH2-terminal kinases (JNKs) and β-actin as the control group. Curcumin has a biphasic effect on SC-NPC proliferation. Lower dosage (0.1, 0.5, 1 µM) of curcumin increased SC-NPC proliferation. However, higher dosage decreased SC-NPC proliferation. Also, curcumin stimulates proliferation of SC-NPCs via the MAP kinase signaling pathway, especially involving the p-ERK and p-38 protein. The p-ERK protein and p38 protein levels varied depending on curcumin dosage (0.5 and 1 µM, p<0.05). Curcumin can stimulate proliferation of SC-NPCs via ERKs and the p38 signaling pathway in low concentrations.
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影响因子：
4.3
作者：
Suh, Hyun-Woo;Kang, Seongman;Kwon, Ki-Sun
通讯作者：
Kwon, Ki-Sun
影响因子：
5.3
作者：
Liu, RH;Morassutti, DJ;Magnuson, DSK
通讯作者：
Magnuson, DSK
DOI：
10.3390/molecules16064567
发表时间：
2011-06-03
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Basnet P;Skalko-Basnet N
通讯作者：
Skalko-Basnet N
影响因子：
1.7
作者：
Liu, Zhi-Qiang;Xing, Shan-Shan;Zhang, Wei
通讯作者：
Zhang, Wei
影响因子：
21.1
作者：
Mujoo, Kalpana;Nikonoff, Lubov E.;Murad, Ferid
通讯作者：
Murad, Ferid