Toward accurate cerebral blood flow estimation in mice after accounting for anesthesia.
Toward accurate cerebral blood flow estimation in mice after accounting for anesthesia.
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DOI：
10.3389/fphys.2023.1169622
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发表时间：
2023
影响因子：
4
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中科院分区：
文献类型：
--
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目的：考虑麻醉效应，提高小鼠脑血流量(CBF)测量的准确性。方法：在4种不同麻醉方案下，同时测量呼吸频率(RR)和心率(HR)，观察脑血流量与麻醉剂量和时间的关系。采用相位对比(PC)磁共振技术测量脑血流量。RR用小鼠监测系统(MouseOX)评估，HR用超短TE MRI序列测定。动态记录19只小鼠的CBF、RR和HR，时间分辨率为1分钟。用线性回归模型研究CBF、麻醉剂量、RR和HR之间的关系。结果：CBF、RR和HR均表现出明显的麻醉剂量依赖性(p<；0.0001)。然而，剂量本身不足以解释生理参数的变化，因为即使是恒定的剂量，它们也表现出随时间的变化。RR和HR一起可以解释CBF测量中52.6%的变异，大于麻醉剂量所解释的变异量(32.4%)。基于多参数回归结果，提出了一种修正小鼠脑血流量测量中麻醉效应的模型。我们还报告了小鼠的清醒状态脑血流量为142.0±8.8mL100微克/分钟，与模型预测值一致。结论：采用考虑呼吸频率、心率和麻醉剂量的校正模型可以提高小鼠脑血流量测量的准确性。
Purpose: To improve the accuracy of cerebral blood flow (CBF) measurement in mice by accounting for the anesthesia effects. Methods: The dependence of CBF on anesthesia dose and time was investigated by simultaneously measuring respiration rate (RR) and heart rate (HR) under four different anesthetic regimens. Quantitative CBF was measured by a phase-contrast (PC) MRI technique. RR was evaluated with a mouse monitoring system (MouseOX) while HR was determined using an ultrashort-TE MRI sequence. CBF, RR, and HR were recorded dynamically with a temporal resolution of 1 min in a total of 19 mice. Linear regression models were used to investigate the relationships among CBF, anesthesia dose, RR, and HR. Results: CBF, RR, and HR all showed a significant dependence on anesthesia dose (p < 0.0001). However, the dose in itself was insufficient to account for the variations in physiological parameters, in that they showed a time-dependent change even for a constant dose. RR and HR together can explain 52.6% of the variations in CBF measurements, which is greater than the amount of variance explained by anesthesia dose (32.4%). Based on the multi-parametric regression results, a model was proposed to correct the anesthesia effects in mouse CBF measurements, specifically . We also reported awake-state CBF in mice to be 142.0 ± 8.8 mL/100 g/min, which is consistent with the model-predicted value. Conclusion: The accuracy of CBF measurement in mice can be improved by using a correction model that accounts for respiration rate, heart rate, and anesthesia dose.
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