Enhanced waterlogging tolerance in barley by manipulation of expression of the N-end rule pathway E3 ligase PROTEOLYSIS6.
Enhanced waterlogging tolerance in barley by manipulation of expression of the N-end rule pathway E3 ligase PROTEOLYSIS6.
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DOI：
10.1111/pbi.12334
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发表时间：
2016-01
影响因子：
13.8
通讯作者：
Holdsworth MJ
中科院分区：
文献类型：
--
作者：
Mendiondo GM;Gibbs DJ;Szurman-Zubrzycka M;Korn A;Marquez J;Szarejko I;Maluszynski M;King J;Axcell B;Smart K;Corbineau F;Holdsworth MJ
提高作物对洪水期间低氧（缺氧）的耐受性是粮食安全的一个关键目标。拟南芥（Arabidopsis thaliana（L.）嘿，靶向蛋白水解的N末端规则途径通过调节VII族乙烯反应因子（ERFVII）转录因子的稳定性来控制植物对缺氧的反应，所述稳定性由氨基末端（Nt）-半胱氨酸（Cys）的氧化状态控制。在这里，我们表明，大麦（Hordeum vulgare L.）ERFVII BERF 1是体外N末端规则途径的底物。此外，我们表明Nt-Cys在体内充当缺氧的传感器，因为氧传感器报告蛋白MCGGAIL-GUS的稳定性在淹水的转基因植物中增加。转基因RNAi大麦植物的N-末端规则途径N-识别蛋白E3连接酶PROTEOLYSIS 6（HvPRT 6）的表达减少，表现出低氧相关基因的表达增加和种子萌发表型改变。此外，在应对水涝时，转基因植物表现出持续的生物量、提高的产量、叶绿素的保留和缺氧相关基因的诱导增强。HvPRT 6 RNAi植物还显示出响应持续黑暗的叶绿素降解减少，这通常与淹水条件有关。含有HvPRT 6突变等位基因的大麦靶向诱导基因组局部损伤（TILLING）系也显示出与RNAi系相似的低氧相关基因和表型的表达增加。我们的结论是，N末端规则途径代表了植物育种的重要目标，以提高大麦和其他谷物的耐涝性。
Increased tolerance of crops to low oxygen (hypoxia) during flooding is a key target for food security. In Arabidopsis thaliana (L.) Heynh., the N‐end rule pathway of targeted proteolysis controls plant responses to hypoxia by regulating the stability of group VII ethylene response factor (ERFVII) transcription factors, controlled by the oxidation status of amino terminal (Nt)‐cysteine (Cys). Here, we show that the barley (Hordeum vulgare L.) ERFVII BERF1 is a substrate of the N‐end rule pathway in vitro. Furthermore, we show that Nt‐Cys acts as a sensor for hypoxia in vivo, as the stability of the oxygen‐sensor reporter protein MCGGAIL‐GUS increased in waterlogged transgenic plants. Transgenic RNAi barley plants, with reduced expression of the N‐end rule pathway N‐recognin E3 ligase PROTEOLYSIS6 (HvPRT6), showed increased expression of hypoxia‐associated genes and altered seed germination phenotypes. In addition, in response to waterlogging, transgenic plants showed sustained biomass, enhanced yield, retention of chlorophyll, and enhanced induction of hypoxia‐related genes. HvPRT6 RNAi plants also showed reduced chlorophyll degradation in response to continued darkness, often associated with waterlogged conditions. Barley Targeting Induced Local Lesions IN Genomes (TILLING) lines, containing mutant alleles of HvPRT6, also showed increased expression of hypoxia‐related genes and phenotypes similar to RNAi lines. We conclude that the N‐end rule pathway represents an important target for plant breeding to enhance tolerance to waterlogging in barley and other cereals.
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影响因子：
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