Reduced colonic mucin degradation in breastfed infants colonized by Bifidobacterium longum subsp. infantis EVC001.
Reduced colonic mucin degradation in breastfed infants colonized by Bifidobacterium longum subsp. infantis EVC001.
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DOI：
10.1002/2211-5463.12516
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发表时间：
2018-10
期刊：
影响因子：
2.6
通讯作者：
Frese SA
中科院分区：
文献类型：
--
作者：
Karav S;Casaburi G;Frese SA
粘蛋白糖蛋白在保护肠道上皮中发挥重要作用，通过阻止肠道微生物与肠道上皮直接接触，同时允许小分子从管腔扩散到上皮。粘蛋白糖萼可被肠道细菌（如拟杆菌和阿克曼氏杆菌）降解，但其他细菌（如长双歧杆菌亚种）可降解。婴儿，不能食用粘蛋白聚糖。将非靶向质谱谱与微生物组谱进行比较，以评估不同的肠道微生物组如何影响结肠粘蛋白降解。比较了9名婴儿EVC001婴儿双歧杆菌定植的样本和10名婴儿EVC001婴儿EVC001定植的高水平溶黏液分类群（对照组），包括拟杆菌。以前使用非靶向纳米-高效液相色谱-芯片/飞行时间质谱法检测和定量源自结肠粘蛋白的聚糖。来自对照婴儿的结肠粘蛋白衍生的O -聚糖占总聚糖结构池的37.68%（±3.14% SD），而在婴儿B. EVC001样本中，结肠粘蛋白衍生的O -聚糖仅占总聚糖结构池的1.78%（±0.038% SD）。这些结肠粘蛋白衍生的O -聚糖在总聚糖池中的相对丰度在对照组中较高，为26.98%（±8.48% SD），相对于b.s infantis定植的婴儿，为1.68%（±1.12% SD）。关键类群如拟杆菌科（Bacteroidaceae）与这些结构丰度呈显著正相关，双歧杆菌科（bifidobacteraceae）与这些结构丰度呈显著负相关。这些结果表明，婴儿b型婴儿定植可能减少结肠聚糖降解，并有助于维持婴儿胃肠道屏障功能。
Mucin glycoproteins play an important role in protecting the gut epithelium by keeping gut microbes from direct contact with the gut epithelium while allowing for diffusion of small molecules from the lumen to the epithelium. The mucin glycocalyx can be degraded by gut bacteria such as Bacteroides and Akkermansia, but other bacteria, such as Bifidobacterium longum subsp. Infantis, cannot consume mucin glycans. Untargeted mass spectrometry profiles were compared to microbiome profiles to assess how different gut microbiomes affect colonic mucin degradation. Samples obtained from nine infants colonized by Bifidobacterium infantis EVC001 and from 10 infants colonized by higher levels of mucolytic taxa (controls), including Bacteroides, were compared. Previously performed untargeted nano‐high‐performance liquid chromatography‐chip/time‐of‐flight mass spectrometry was used to detect and quantify glycans originating from colonic mucin. Colonic mucin‐derived O‐glycans from control infants composed 37.68% (± 3.14% SD) of the total glycan structure pool, whereas colonic mucin‐derived O‐glycans made up of only 1.78% (± 0.038% SD) of the total in B. infantis EVC001 samples. The relative abundance of these colonic mucin‐derived O‐glycans in the total glycan pool was higher among control, 26.98% (± 8.48% SD), relative to B. infantis‐colonized infants, 1.68% (± 1.12% SD). Key taxa, such as Bacteroidaceae, were significantly and positively correlated with the abundance of these structures, while Bifidobacteriaceae were significantly and negatively associated with these structures. These results suggest that colonization of infants by B. infantis may diminish colonic glycan degradation and help maintain barrier function in the gastrointestinal tract of infants.
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影响因子：
30.3
作者：
Marcobal A;Barboza M;Sonnenburg ED;Pudlo N;Martens EC;Desai P;Lebrilla CB;Weimer BC;Mills DA;German JB;Sonnenburg JL
通讯作者：
Sonnenburg JL
影响因子：
4.4
作者：
Karav, Sercan;Le Parc, Annabelle;Mills, David A.
通讯作者：
Mills, David A.
影响因子：
4.1
作者：
Robbe, C;Capon, C;Michalski, JC
通讯作者：
Michalski, JC
影响因子：
4.6
作者：
Garrido D;Ruiz-Moyano S;Lemay DG;Sela DA;German JB;Mills DA
通讯作者：
Mills DA
影响因子：
3.7
作者：
Tailford LE;Crost EH;Kavanaugh D;Juge N
通讯作者：
Juge N