Dual lineage-specific expression of Sox17 during mouse embryogenesis.
Dual lineage-specific expression of Sox17 during mouse embryogenesis.
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Sox17 在小鼠胚胎发生过程中的双谱系特异性表达。
DOI：
10.1002/stem.1192
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发表时间：
2012-10
期刊：
影响因子：
5.2
通讯作者：
Magnuson, Mark A.
中科院分区：
文献类型：
--
作者：
Choi, Eunyoung;Kraus, Marine R-C.;Lemaire, Laurence A.;Yoshimoto, Momoko;Vemula, Sasidhar;Potter, Leah A.;Manduchi, Elisabetta;Stoeckert, Christian J., Jr.;Grapin-Botton, Anne;Magnuson, Mark A.
Sox 17对于内胚层发育和胎儿造血干细胞（HSC）维持至关重要。虽然众所周知内胚层来源的器官起源于Sox 17表达细胞，但不太确定胎儿HSC是否也起源于Sox 17表达细胞。通过产生一个Sox 17 GFPCre等位基因，并使用它来评估Sox 17表达细胞在胚胎发生过程中的命运，我们证实，内胚层和一部分永久造血细胞来源于Sox 17阳性细胞。在E9.5之前，Sox 17的表达限于内胚层谱系。然而，在E9.5，Sox 17在主动脉旁内脏胸膜（P-Sp）区域的内皮细胞（EC）中表达，其有助于在后期阶段形成HSC。使用FACS与RNA-Seq一起确定两个细胞群的基因表达谱，证实了在E9.5表达Sox 17的两个不同祖细胞群的鉴定。有趣的是，该分析揭示了胚胎发生过程中Sox 17 mRNA的RNA加工差异。综上所述，这些结果表明，Sox 17表达在祖细胞来源于两个不同的胚层，进一步证明了该基因的复杂的表达模式，并建议谨慎使用Sox 17作为谱系特异性标记。
Sox17 is essential for both endoderm development and fetal hematopoietic stem cell (HSC) maintenance. While endoderm-derived organs are well known to originate from Sox17-expressing cells it is less certain whether fetal HSCs also originate from Sox17-expressing cells. By generating a Sox17GFPCre allele and using it to assess the fate of Sox17-expressing cells during embryogenesis we confirmed that both endodermal and a part of definitive hematopoietic cells are derived from Sox17-positive cells. Prior to E9.5 the expression of Sox17 is restricted to the endoderm lineage. However, at E9.5 Sox17 is expressed in the endothelial cells (ECs) at the para-aortic splanchnopleural (P-Sp) region that contribute to the formation of HSCs at a later stage. The identification of two distinct progenitor cell populations that express Sox17 at E9.5 was confirmed using FACS together with RNA-Seq to determine the gene expression profiles of the two cell populations. Interestingly, this analysis revealed differences in the RNA processing of the Sox17 mRNA during embryogenesis. Taken together, these results indicate that Sox17 is expressed in progenitor cells derived from two different germ layers, further demonstrating the complex expression pattern of this gene and suggesting caution when using Sox17 as a lineage-specific marker.
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影响因子：
1.5
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Woodland, HR