Cell type-dependent induction of DNA damage by 1800 MHz radiofrequency electromagnetic fields does not result in significant cellular dysfunctions.
Cell type-dependent induction of DNA damage by 1800 MHz radiofrequency electromagnetic fields does not result in significant cellular dysfunctions.
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1800 MHz 射频电磁场对细胞类型依赖性 DNA 损伤的诱导不会导致明显的细胞功能障碍
DOI：
10.1371/journal.pone.0054906
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Xu Z
中科院分区：
文献类型：
--
作者：
Xu S;Chen G;Chen C;Sun C;Zhang D;Murbach M;Kuster N;Zeng Q;Xu Z
背景 尽管 IARC 澄清射频电磁场 (RF-EMF) 可能是人类致癌物，但由于结果不一致，关于其健康影响的争论仍在继续。基因毒性效应被认为是确定环境因素是否致癌的黄金标准，但目前可用的射频电磁场数据仍然存在争议。作为一种环境刺激，RF-EMF 对细胞 DNA 的影响可能是微妙的。因此，需要更灵敏的方法和系统的研究策略来评估其遗传毒性。目的 采用更灵敏的 γH2AX 病灶形成方法来确定 RF-EMF 是否会诱导 DNA 损伤，以及该效应是否具有细胞类型依赖性；并研究 RF-EMF 是否确实增加 γH2AX 病灶形成的生物学后果。方法将6种不同类型的细胞间歇性暴露于比吸收率3.0 W/kg的GSM 1800 MHz RF-EMF下1 h或24 h，然后用抗γH2AX抗体进行免疫染色。通过彗星和 TUNEL 测定、流式细胞术和细胞生长测定进一步探讨了 γH2AX 升高的细胞类型的生物学后果。结果暴露于 RF-EMF 24 小时显着诱导中国仓鼠肺细胞和人皮肤成纤维细胞 (HSF) 中 γH2AX 灶的形成，但对其他细胞没有影响。然而，HSF 细胞中 RF-EMF 升高的 γH2AX 灶形成并没有导致可检测到的 DNA 碎片、可持续的细胞周期停滞、细胞增殖或活力变化。 RF-EMF 暴露会轻微但不会显着增加细胞 ROS 水平。结论 RF-EMF 以细胞类型依赖性方式诱导 DNA 损伤，但 HSF 细胞中 γH2AX 灶形成的增加不会导致明显的细胞功能障碍。
Background Although IARC clarifies radiofrequency electromagnetic fields (RF-EMF) as possible human carcinogen, the debate on its health impact continues due to the inconsistent results. Genotoxic effect has been considered as a golden standard to determine if an environmental factor is a carcinogen, but the currently available data for RF-EMF remain controversial. As an environmental stimulus, the effect of RF-EMF on cellular DNA may be subtle. Therefore, more sensitive method and systematic research strategy are warranted to evaluate its genotoxicity. Objectives To determine whether RF-EMF does induce DNA damage and if the effect is cell-type dependent by adopting a more sensitive method γH2AX foci formation; and to investigate the biological consequences if RF-EMF does increase γH2AX foci formation. Methods Six different types of cells were intermittently exposed to GSM 1800 MHz RF-EMF at a specific absorption rate of 3.0 W/kg for 1 h or 24 h, then subjected to immunostaining with anti-γH2AX antibody. The biological consequences in γH2AX-elevated cell type were further explored with comet and TUNEL assays, flow cytometry, and cell growth assay. Results Exposure to RF-EMF for 24 h significantly induced γH2AX foci formation in Chinese hamster lung cells and Human skin fibroblasts (HSFs), but not the other cells. However, RF-EMF-elevated γH2AX foci formation in HSF cells did not result in detectable DNA fragmentation, sustainable cell cycle arrest, cell proliferation or viability change. RF-EMF exposure slightly but not significantly increased the cellular ROS level. Conclusions RF-EMF induces DNA damage in a cell type-dependent manner, but the elevated γH2AX foci formation in HSF cells does not result in significant cellular dysfunctions.
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影响因子：
1.6
作者：
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DOI：
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