Characterization of Lymphocyte Subsets in Lymph Node and Spleen Sections in Fatal Pediatric Malaria.
Characterization of Lymphocyte Subsets in Lymph Node and Spleen Sections in Fatal Pediatric Malaria.
复制标题
致死性小儿疟疾淋巴结和脾切片中淋巴细胞亚群的特征。
DOI：
10.3390/pathogens11080851
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发表时间：
2022-07-28
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
次级淋巴组织在人对恶性疟原虫感染的免疫反应中起主要作用。先前的研究表明，急性恶性疟疾与细胞免疫系统的显著扰动有关，其特征是循环T细胞亚群的频率和绝对数量降低。对于这一现象，有两种可能的解释是T细胞的暂时迁移，可能是通过浸润到次级淋巴组织，或者它们通过细胞凋亡而永久丢失。本研究旨在确定马拉维儿童恶性疟疾感染急性期淋巴结和脾脏中积聚的淋巴细胞亚群的表型，并验证淋巴细胞在急性感染期间迁移到淋巴组织的假设。我们对马拉维两种常见临床形式严重疟疾死亡儿童的组织切片进行染色，即严重疟疾贫血（SMA, n = 1）和脑型疟疾（CM, n = 3），并使用非疟疾败血症死亡儿童的组织切片作为对照（n = 2）。与SMA患者相比，CM患者的淋巴结和脾脏组织（红髓）切片具有更高的T细胞（CD4+和CD8+）百分比。在后者中，我们观察到淋巴结中CD20+ B细胞的百分比高于CM患者，而在脾脏中观察到相反的情况。与SMA患者的组织切片相比，CM患者的淋巴结和脾脏切片的CD69+和CD45RO+细胞的百分比都有所增加。这些结果支持了淋巴细胞向脾脏和淋巴结转移可能导致急性CM泛淋巴细胞减少的假设。
Secondary lymphoid tissues play a major role in the human immune response to P. falciparum infection. Previous studies have shown that acute falciparum malaria is associated with marked perturbations of the cellular immune system characterized by lowered frequency and absolute number of circulating T cell subsets. A temporary relocation of T cells, possibly by infiltration to secondary lymphoid tissue, or their permanent loss through apoptosis, are two proposed explanations for this observation. We conducted the present study to determine the phenotype of lymphocyte subsets that accumulate in the lymph node and spleen during acute stages of falciparum malaria infection in Malawian children, and to test the hypothesis that lymphocytes are relocated to lymphoid tissues during acute infection. We stained tissue sections from children who had died of the two common clinical forms of severe malaria in Malawi, namely severe malarial anemia (SMA, n = 1) and cerebral malaria (CM, n = 3), and used tissue sections from pediatric patients who had died of non-malaria sepsis (n = 2) as controls. Both lymph node and spleen tissue (red pulp) sections from CM patients had higher percentages of T cells (CD4+ and CD8+) compared to the SMA patient. In the latter, we observed a higher percentage of CD20+ B cells in the lymph nodes compared to CM patients, whereas the opposite was observed in the spleen. Both lymph node and spleen sections from CM patients had increased percentages of CD69+ and CD45RO+ cells compared to tissue sections from the SMA patient. These results support the hypothesis that the relocation of lymphocytes to spleen and lymph node may contribute to the pan-lymphopenia observed in acute CM.
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影响因子：
5.5
作者：
Ghosh D;Stumhofer JS
通讯作者：
Stumhofer JS
DOI：
10.4269/ajtmh.1994.51.372
发表时间：
1994-09-01
影响因子：
3.3
作者：
ELHASSAN, IM;HVIID, L;THEANDER, TG
通讯作者：
THEANDER, TG
影响因子：
3.1
作者：
Hviid, L;Kurtzhals, JAL;Theander, TG
通讯作者：
Theander, TG
DOI：
10.1016/s0167-5699(05)80020-9
发表时间：
1991-03-01
期刊：
IMMUNOPARASITOLOGY TODAY-A COMBINED ISSUE OF IMMUNOLOGY TODAY AND PARASITOLOGY TODAY
影响因子：
--
作者：
DAY, KP;MARSH, K
通讯作者：
MARSH, K
影响因子：
3.1
作者：
Urban, BC;Hien, TT;Roberts, DJ
通讯作者：
Roberts, DJ