EFFECTS OF VITAMIN E SUPPLEMENTATION ON SERUM HORMONES AND GENE EXPRESSION OF ANTI-SEASON BREEDING XINGGUO GREY GEESE (ANSER CYGNOIDES)

EFFECTS OF VITAMIN E SUPPLEMENTATION ON SERUM HORMONES AND GENE EXPRESSION OF ANTI-SEASON BREEDING XINGGUO GREY GEESE (ANSER CYGNOIDES)
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补充维生素E对反季节养殖兴国灰鹅血清激素及基因表达的影响

DOI:
10.1590/1806-9061-2018-0892
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发表时间:
2019
影响因子:
1.1
通讯作者:
Liu Y.
Liu Y.
中科院分区:
农林科学4区
文献类型:
--
作者:
Yin L.;Ran J.;Lian T.;Yang C.;Li S.;Liu Y.

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本试验旨在分析饲粮中添加维生素E对反季节种鹅产蛋性能、血清生殖激素浓度及卵巢和卵泡基因表达的影响。210只反季种鹅分为7个处理,6个重复。各组饲喂不同维生素E (DL-α-乙酸生育酚)含量(0、10、20、40、80、160、320mg/kg)的饲粮。我们观察到,10和80 mg/kg剂量组的产蛋率和产蛋率显著提高,而蛋重在320 mg/kg组最高。同时,添加80 mg/kg维生素E显著提高了血清促卵泡激素(FSH)、黄体生成素(LH)和雌二醇(E2)浓度(p<0.05)。饲粮中添加维生素E可显著提高80mg/kg剂量组FSHR、LHR和ESR1 mRNA表达量,10和20mg/kg剂量组PRLR mRNA表达量显著升高(p<0.05)。结果表明,在维生素E添加剂量为40和80 mg/kg时,卵巢、SWF、LWF和SYF中Dicer mRNA的表达量分别增加。由此可见,饲粮中添加维生素E可提高蛋鸡的产蛋性能、血浆生殖激素水平和卵巢中生殖激素受体基因mRNA表达量,以及卵巢、SWF、LWF和SYF中Dicer mRNA表达量。由此得出,在饲料中添加80 mg/kg维生素E可提高反季节繁殖兴国灰鹅的生育能力。
The objective of this study was to analyse the impact of dietary vitamin E supplementation on laying performance, serum reproductive hormones concentration and gene expression in ovary and follicles of anti-season breeding goose. A total of 210 anti-season breeding geese were divided into seven treatments with six replications. Each group was supplied with diets containing different vitamin E (DL-α-tocopherol acetate) contents (0, 10, 20, 40, 80, 160, 320mg/kg). We observed that the egg production and laying rate improved significantly at doses of 10 and 80 mg/kg, while the highest egg weight appeared in the 320 mg/kg group. Meantime, 80 mg/kg of vitamin E supplementation significantly improved the concentration of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) in serum (p<0.05). Dietary vitamin E supplementation significantly enhanced mRNA expression of FSHR, LHR and ESR1 at a dose of 80mg/kg, while PRLR increased at doses of 10 and 20mg/kg (p<0.05). It was found that the mRNA expression of Dicer increased at doses of 40 and 80 mg/kg of vitamin E supplementation in the ovary, SWF, LWF and SYF, respectively. Thence, Dietary vitamin E supplementation could improve egg laying performance, plasma reproductive hormones and the mRNA expression of reproductive hormone receptor genes in ovary, as well as the mRNA expression of Dicer in ovary, SWF, LWF and SYF. It was supposed that 80 mg/kg of vitamin E supplementation in dietary was appropriate to improve the fertility of anti-season breeding Xingguo grey goose.
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