Protective Effect of Spore Powder of Antrodia camphorata ATCC 200183 on CCl(4)-Induced Liver Fibrosis in Mice.
Protective Effect of Spore Powder of Antrodia camphorata ATCC 200183 on CCl(4)-Induced Liver Fibrosis in Mice.
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牛樟芝孢子粉 ATCC 200183 对 CCl4 所致小鼠肝纤维化的保护作用
DOI：
10.3390/nu12092778
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发表时间：
2020-09-11
期刊：
影响因子：
5.9
通讯作者：
Xu ZH
中科院分区：
文献类型：
--
作者：
Ren Y;Li HX;Zhou L;Lu ZM;Shi J;Geng Y;Xu ZH
肝纤维化是过量细胞外基质在肝内弥漫性沉积的病理过程，导致各种慢性肝病。治疗肝纤维化的高效低毒药物尚不多见。牛樟芝具有抗氧化、抗病毒、抗肿瘤、抗炎等作用，在人群中已被用于治疗肝脏疾病。然而，A.然而，关于其孢子及其背后的机制还没有研究。本研究评价了灵芝孢子粉的保肝作用。黄芪（SP，100 mg/kg/天或200 mg/kg/天）对四氯化碳（CCl 4）诱导的小鼠肝纤维化的影响。与CCl 4组相比，SP组降低血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）活性。肝组织羟脯氨酸（Hyp）含量明显降低。SP可改善H&E、天狼星红和Masson染色的细胞损伤，减少胶原沉积。此外，SP还下调α-SMA和Col 1的mRNA水平以及α-平滑肌肌动蛋白（α-SMA）、I型胶原（Col 1）、肿瘤坏死因子α（TNF-α）、Toll样受体4（TLR 4）和核因子κB（NF-κB）p65的蛋白表达。综上所述，SP对肝纤维化具有改善作用，可能是通过抑制肝星状细胞的活化，减少细胞外基质的合成来实现的。
Liver fibrosis is a pathological process with intrahepatic diffused deposition of the excess extracellular matrix, which leads to various chronic liver diseases. Drugs with high efficacy and low toxicity for liver fibrosis are still unavailable. Antrodia camphorata has antioxidant, antivirus, antitumor and anti-inflammation roles, and has been used to treat liver diseases in the population. However, the hepatoprotective effects of A. camphorata spores and the mechanisms behind it have not been investigated. In this study, we evaluate the hepatoprotective effect of spore powder of A. camphorata (SP, 100 mg/kg/day or 200 mg/kg/day) on carbon tetrachloride (CCl4)-induced liver fibrosis in mice. SP groups reduced serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities compared with the CCl4 group. SP also showed a decrease in hydroxyproline (Hyp) content in liver tissues. SP improved cell damage and reduced collagen deposition by H&E, Sirius red and Masson staining. Furthermore, SP down-regulated the mRNA levels of α-SMA and Col 1, and the protein expression of α-smooth muscle actin (α-SMA), collagen I (Col 1), tumor necrosis factor alpha (TNF-α), toll like receptor 4 (TLR4) and nuclear factor-Κb (NF-κB) p65. In summary, SP has an ameliorative effect on hepatic fibrosis, probably by inhibiting the activation of hepatic stellate cells, reducing the synthesis of extracellular matrix.
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影响因子：
5.9
作者：
Pan Y;Long X;Yi R;Zhao X
通讯作者：
Zhao X
影响因子：
6.1
作者：
Song, TY;Yen, GC
通讯作者：
Yen, GC
DOI：
10.1016/j.bbrc.2010.04.094
发表时间：
2010-05-28
影响因子：
3.1
作者：
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通讯作者：
Weiskirchen, Ralf
影响因子：
8.8
作者：
Tsai, Ming-Chi;Song, Tuzz-Ying;Yen, Gow-Chin
通讯作者：
Yen, Gow-Chin
影响因子：
5.9
作者：
Go J;Kim JE;Koh EK;Song SH;Sung JE;Lee HA;Lee YH;Lim Y;Hong JT;Hwang DY
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