Polyphenols in Liubao Tea Can Prevent CCl₄-Induced Hepatic Damage in Mice through Its Antioxidant Capacities.
Polyphenols in Liubao Tea Can Prevent CCl₄-Induced Hepatic Damage in Mice through Its Antioxidant Capacities.
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DOI：
10.3390/nu10091280
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发表时间：
2018-09-10
期刊：
影响因子：
5.9
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Pan Y;Long X;Yi R;Zhao X
研究了六堡茶多酚类物质对四氯化碳（CCl 4）致小鼠肝损伤的预防作用。小鼠首先接受PLT治疗，然后使用10 mL/kg CCl 4诱导肝损伤。检测肝脏和血清指标，以及肝组织中相关信使RNA（mRNA）和蛋白质的表达水平。结果表明，PLT可降低肝损伤小鼠的肝脏质量和各项指标。PLT还下调肝损伤小鼠血清中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、甘油三酯（TG）和丙二醛（MDA），上调超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）。PLT还可降低肝损伤小鼠血清中白细胞介素-6（IL-6）、白细胞介素-12（IL-12）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）细胞因子的水平。病理形态学观察也显示PLT减轻了CCl 4诱导的肝组织中心静脉分化和肝细胞损伤。此外，qPCR和Western blot也证实PLT上调肝组织中Gu/Zn-SOD、Mn-SOD、过氧化氢酶（CAT）、GSH-Px和B细胞抑制剂-α（IκB-α）中κ轻链多肽基因增强子核因子的mRNA和蛋白表达，下调活化B细胞的环氧化酶2（考克斯-2）和κ轻链增强子核因子（NF-κB）的表达。同时，PLT还能上调肝损伤小鼠磷酸化（p）-NF-κB p65和细胞色素P450还原酶蛋白的表达。PLT的主要成分包括没食子酸、儿茶素、咖啡因、表儿茶素（EC）、表没食子儿茶素没食子酸酯（EGCG）、没食子儿茶素没食子酸酯（GCG）和表儿茶素没食子酸酯（ECG）等，它们可能具有广泛的生物活性。因此，PLT对CCl 4诱导的肝损伤具有预防作用，这与水飞蓟素相似。
The present study investigated the preventive effect of polyphenols in Liubao tea (PLT) on carbon tetrachloride (CCl4)-induced liver injury in mice. The mice were initially treated with PLT, followed by induction of liver injury using 10 mL/kg CCl4. Then liver and serum indices, as well as the expression levels of related messenger RNAs (mRNAs) and proteins in liver tissues were measured. The results showed that PLT reduces the liver quality and indices of mice with liver injury. PLT also downregulates aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglycerides (TGs), and malondialdehyde (MDA), and upregulates superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the sera of mice with liver injury. PLT also reduces serum levels of interleukin-6 (IL-6), interleukin-12 (IL-12), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) cytokines in mice with liver injury. Pathological morphological observation also shows that PLT reduces CCl4-induced central venous differentiation of liver tissues and liver cell damage. Furthermore, qPCR and Western blot also confirm that PLT upregulates the mRNA and protein expressions of Gu/Zn-SOD, Mn-SOD, catalase (CAT), GSH-Px, and nuclear factor of κ-light polypeptide gene enhancer in B-cells inhibitor-α (IκB-α) in liver tissues, and downregulates the expression of cyclooxygenase 2 (COX-2) and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB). Meanwhile, PLT also raised the phosphorylated (p)-NF-κB p65 and cytochrome P450 reductase protein expression in liver injury mice. The components of PLT include gallic acid, catechin, caffeine, epicatechin (EC), epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), and epicatechin gallate (ECG), which possibly have a wide range of biological activities. Thus, PLT imparts preventive effects against CCl4-induced liver injury, which is similar to silymarin.
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