The complete mitochondrial genome of the Chinese Daphnia pulex (Cladocera, Daphniidae).
The complete mitochondrial genome of the Chinese Daphnia pulex (Cladocera, Daphniidae).
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中国水蚤（枝角类，水蚤科）完整线粒体基因组
DOI：
10.3897/zookeys.615.8581
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发表时间：
2016
期刊：
影响因子：
1.3
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Geng X;Cheng R;Xiang T;Deng B;Wang Y;Deng D;Zhang H
摘要蚤状蚤在淡水生态系统中起着重要作用。本研究首次对中国巢湖蚤状蚤线粒体基因组进行了全序列测定。它是使用长PCR方法和具有属特异性引物的引物步移测序策略完成的。发现有丝分裂基因组的长度为15，306 bp。它包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和一个典型的控制区。这项研究揭示了总的A+T含量为64.50%。所有22个典型的动物tRNA基因都具有典型的三叶草结构，除了trnS1，其DHU臂简单地形成一个环。小rRNA和大rRNA的长度分别为744 bp和1，313 bp。A+ T富集区长度为723 bp，比北美物种的A + T富集区（689 bp）长。在结构和组成方面，发现中国和北美蚤状蚤之间有许多相似之处。
Abstract Daphnia pulex has played an important role in fresh-water ecosystems. In this study, the complete mitochondrial genome of Daphnia pulex from Chaohu, China was sequenced for the first time. It was accomplished using long-PCR methods and a primer-walking sequencing strategy with genus-specific primers. The mitogenome was found to be 15,306 bp in length. It contained 13 protein-coding genes, two rRNA genes, 22 tRNA genes and a typical control region. This research revealed an overall A+T content of 64.50%. All of the 22 typical animal tRNA genes had a classical clover-leaf structure except for trnS1, in which its DHU arm simply formed a loop. The lengths of small and large rRNA were 744 bp and 1,313 bp, respectively. The A+T-rich region was 723 bp in length, which is longer than that from the North American species (689 bp). In terms of structure and composition, many similarities were found between the Chinese and North American Daphnia pulex.
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影响因子：
1.3
作者：
Wang P;Yang HF;Zhou WC;Hwang CC;Zhang WH;Qian ZX
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影响因子：
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影响因子：
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作者：
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通讯作者：
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