Heat shock proteins contribute to mosquito dehydration tolerance.
Heat shock proteins contribute to mosquito dehydration tolerance.
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DOI：
10.1016/j.jinsphys.2009.09.012
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发表时间：
2010-02
影响因子：
2.2
通讯作者：
Denlinger, David L.
中科院分区：
文献类型：
--
作者：
Benoit, Joshua B.;Lopez-Martinez, Giancarlo;Phillips, Zachary P.;Patrick, Kevin R.;Denlinger, David L.
本研究探讨了热休克蛋白转录本，热休克蛋白70和热休克蛋白90，脱水应激在三种蚊子，埃及伊蚊，冈比亚按蚊和尖音库蚊。我们首先定义了每个物种的成年女性的水平衡属性，监测响应于脱水的热休克蛋白的转录本的表达，然后敲低的转录本的表达使用RNA干扰（RNAi），以评估潜在的功能的热休克蛋白在维持水平衡。这三个物种的完全水合的雌性含有几乎相同的水量（66-68%），但水分流失率在物种之间存在差异，A。埃及伊蚊的失水速率最低（2.6%/h），尖音线虫次之（3.3%/h），埃及伊蚊最低（2.6%/h），尖音线虫次之（3.3%/h）。冈比亚（5.1%/h）。在所有三种物种中，水只能被饮用水（或血液）取代。A. aegypti和C. pipiens耐受36%的水分损失，而A.冈比亚更容易受到水分流失的影响，只能容忍身体水分流失29%。脱水诱导了三个物种hsp 70的表达，但只有C. pipiens在再水化期间继续表达该转录物。热休克蛋白90的组成性表达和表达水平保持相当稳定，在脱水和复水，除了表达没有注意到在复水的C。pipiens。注射dsRNA以敲低A中hsp 70（83%降低）和hsp 90（46%降低）的表达。埃及伊蚊没有改变含水量或失水率，但脱水耐受性较低。而不是存活36%的水分损失，女性能够存活只有28%的水分损失响应RNAi针对hsp 70和26%的水分损失时，RNAi针对hsp 90。这些结果表明，这些热休克蛋白在蚊子脱水耐受性中起着关键作用
This study examines the responses of heat shock protein transcripts, Hsp70 and Hsp90, to dehydration stress in three mosquito species, Aedes aegypti, Anopheles gambiae and Culex pipiens. We first defined the water balance attributes of adult females of each species, monitored expression of the hsp transcripts in response to dehydration, and then knocked down expression of the transcripts using RNA interference (RNAi) to evaluate potential functions of the Hsps in maintenance of water balance. Fully hydrated females of all three species contained nearly the same amount of water (66–68%), but water loss rates differed among the species, with A. aegypti having the lowest water loss rate (2.6%/h), followed by C.pipiens (3.3%/h), and A. gambiae (5.1%/h). In all three species water could be replaced only by drinking water (or blood). Both A. aegypti and C. pipiens tolerated a loss of 36% of their body water, but A. gambiae was more vulnerable to water loss, tolerating a loss of only 29% of its body water. Dehydration elicited expression of hsp70 in all three species, but only C. pipiens continued to express this transcript during rehydration. Hsp90 was constitutively expressed and expression levels remained fairly constant during dehydration and rehydration, except expression was not noted during rehydration of C. pipiens. Injection of dsRNA to knock down expression of hsp70 (83% reduction) and hsp90 (46% reduction) in A. aegypti did not alter water content or water loss rates, but the dehydration tolerance was lower. Instead of surviving a 36% water loss, females were able to survive only a 28% water loss in response to RNAi directed against hsp70 and a 26% water loss when RNAi was directed against hsp90. These results indicate a critical function for these Hsps in mosquito dehydration tolerance
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DOI：
10.1073/pnas.0507958102
发表时间：
2005-11-01
影响因子：
11.1
作者：
Robich, RM;Denlinger, DL
通讯作者：
Denlinger, DL
影响因子：
2.2
作者：
Tammariello, SP;Rinehart, JP;Denlinger, DL
通讯作者：
Denlinger, DL
影响因子：
2.8
作者：
Hayward, SAL;Rinehart, JP;Denlinger, DL
通讯作者：
Denlinger, DL
DOI：
10.4269/ajtmh.2005.73.553
发表时间：
2005-09-01
影响因子：
3.3
作者：
Gray, EM;Bradley, TJ
通讯作者：
Bradley, TJ
影响因子：
2.8
作者：
Crooks, Emily;Randolph, Sarah E.
通讯作者：
Randolph, Sarah E.