Breeding response of transcript profiling in developing seeds of Brassica napus.
Breeding response of transcript profiling in developing seeds of Brassica napus.
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DOI：
10.1186/1471-2199-10-49
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发表时间：
2009-05-24
影响因子：
--
通讯作者：
Lu C
中科院分区：
文献类型：
--
作者：
Hu Y;Wu G;Cao Y;Wu Y;Xiao L;Li X;Lu C
在过去的30年里，中国油菜籽品种的升级换代使产量和品质有了很大的提高。随着脂肪酸组成和含油量的选择压力，高芥子酸和低含油量的品种已经被低芥子酸和高含油量的品种所取代。高芥子酸品种中优821及其后代低芥子酸品种中双9是近20年来中国最优秀的油菜品种（B. napus）的两代代表。本文比较了中双9号和中优821在种子发育过程中主要参与脂质生物合成的32个基因的转录谱，以阐明这些基因的转录谱在过去20年中对品质改善的响应。将中优821与其后代中双9进行比较，发现32个基因中FAD3、ACCase、FAE1、GKTP、Caleosin、GAPDH、PEPC等7个基因的转录水平上调了30% ~ 109%。32个基因中，10个（KAS3、β-CT、BcRK6、P450、FatA、Oleosin、FAD6、FatB、α-CT和SUC1）至少下调20%，最多下调50%。在这两个品种中，仅Napin基因占所有32个基因总转录量的75%以上。大部分基因与脂肪酸积累呈显著相关，但ZS9与ZY821的相关性差异显著。KCR2活性越高，两个品种的C16:0、C18:0和C18:2含量越高，ZY821的C22:1和总脂肪酸含量越低，ZS9的C22:1含量越低。本文阐述了油菜种子发育过程中32个基因的转录水平对育种的响应。两个品种表现出相似的转录谱，以Napin基因为主。零芥酸、低硫代葡萄糖苷、高油酸、高含油量、高产的选择压力导致FAD3、ACCase、FAE1、GKTP、Caleosin、GAPDH、PEPC表达量升高，而KAS3、β-CT、BcRK6、P450、FatA、Oleosin、FAD6、FatB、α-CT、SUC1表达量降低。它还导致这些基因在种子发育过程中储存积累的关系发生改变。
The upgrading of rapeseed cultivars has resulted in a substantial improvement in yield and quality in China over the past 30 years. With the selective pressure against fatty acid composition and oil content, high erucic acid- and low oil-content cultivars have been replaced by low erucic acid- and high oil-content cultivars. The high erucic acid cultivar Zhongyou 821 and its descendent, low erucic acid cultivar Zhongshuang 9, are representatives of two generations of the most outstanding Chinese rapeseed cultivars (B. napus) developed the past 2 decades. This paper compares the transcriptional profiles of Zhongshuang 9 and Zhongyou 821 for 32 genes that are principally involved in lipid biosynthesis during seed development in order to elucidate how the transcriptional profiles of these genes responded to quality improvement over the past 20 years. Comparison of the cultivar Zhongyou 821 with its descendent, Zhongshuang 9, shows that the transcriptional levels of seven of the 32 genes were upregulated by 30% to 109%, including FAD3, ACCase, FAE1, GKTP, Caleosin, GAPDH, and PEPC. Of the 32 genes, 10 (KAS3, β-CT, BcRK6, P450, FatA, Oleosin, FAD6, FatB, α-CT and SUC1) were downregulated by at least 20% and most by 50%. The Napin gene alone accounted for over 75% of total transcription from all 32 genes assessed in both cultivars. Most of the genes showed significant correlation with fatty acid accumulation, but the correlation in ZS9 was significantly different from that in ZY821. Higher KCR2 activity is associated with higher C16:0, C18:0, and C18:2 in both cultivars, lower C22:1 and total fatty acid content in ZY821, and lower 18:1 in ZS9. This paper illustrates the response of the transcription levels of 32 genes to breeding in developing rapeseed seeds. Both cultivars showed similar transcription profiles, with the Napin gene predominantly transcribed. Selective pressure for zero erucic acid, low glucosinolate, high oleic acid and high oil content, as well as high yield, resulted in higher FAD3, ACCase, FAE1, GKTP, Caleosin, GAPDH, and PEPC expression levels and lower KAS3, β-CT, BcRK6, P450, FatA, Oleosin, FAD6, FatB, α-CT and SUC1 expression levels. It also resulted in altered relationships between these genes during storage accumulation in seed development.
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影响因子：
7.4
作者：
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通讯作者：
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