Supplemental N-acyl homoserine lactonase alleviates intestinal disruption and improves gut microbiota in broilers challenged by Salmonella Typhimurium.
Supplemental N-acyl homoserine lactonase alleviates intestinal disruption and improves gut microbiota in broilers challenged by Salmonella Typhimurium.
复制标题
补充 N-酰基高丝氨酸内酯酶可减轻受鼠伤寒沙门氏菌感染的肉鸡的肠道破坏并改善肠道微生物群
DOI：
10.1186/s40104-022-00801-4
复制
发表时间：
2023-01-09
影响因子：
7
通讯作者：
Zuo, Jianjun
中科院分区：
文献类型：
--
作者：
Wang, Weiwei;Ou, Jingseng;Ye, Hui;Cao, Qingyun;Zhang, Changming;Dong, Zemin;Feng, Dingyuan;Zuo, Jianjun
关键词：
鼠伤寒沙门氏菌的挑战对鸡肉生产造成巨大损害。 N-酰基高丝氨酸内酯酶 (AHLase) 是一种群体猝灭酶，可能抑制革兰氏阴性菌的生长和毒力。然而，AHLase 是否可以保护鸡免受鼠伤寒沙门氏菌的攻击尚不清楚。本研究旨在评估 AHLase 对受鼠伤寒沙门氏菌攻击的肉鸡生长性能和肠道健康的影响。将240只一日龄雌性杂交肉鸡（817C）随机分为5组（6个重复/组）：阴性对照（NC）、阳性对照（PC）和添加5、10或20 U/g AHLase的PC组。除北卡罗来纳州的鸡外，所有鸡均在 7 至 9 天龄时接受鼠伤寒沙门氏菌攻击。所有与生长和肠道健康相关的参数均在第 10 天和第 14 天测定。添加 AHLase 可以缓解受攻击鸡的体重 (BW) 和平均日增重 (ADG) 的下降 (P < 0.05)，特别是在 10 U/g 时。因此，选择NC、PC和PC加10 U/g AHLase组的样品进行进一步分析。鼠伤寒沙门氏菌攻击第 10 天，肠道形态受损 (P < 0.05)，回肠炎症细胞因子 (IL-1β 和 IL-8) 表达升高 (P < 0.05)，血清二胺氧化酶 (DAO) 活性增加 (P < 0.05)。然而，添加 AHLase 使这些变化正常化。第 10 天的肠道微生物群分析显示，AHLase 逆转了 PC 组中几种有益细菌（例如芽孢杆菌、芽孢杆菌目和乳杆菌目）的减少（P < 0.05），以及某些有害细菌（例如变形菌门、伽玛变形菌门、肠杆菌科和埃希氏菌/志贺氏菌）的增加（P < 0.05）。此外，AHLase诱导的有益菌增加和有害菌减少与回肠IL-1β和IL-8表达以及血清DAO活性的降低基本呈负相关（P < 0.05），而与BW和ADG的增加呈正相关（P < 0.05）。功能预测显示，AHLase 消除了鼠伤寒沙门氏菌诱导的某些致病性相关途径的上调（P < 0.05），例如脂多糖生物合成、志贺氏菌病、上皮细胞的细菌入侵和肠道微生物群的致病性大肠杆菌感染。补充 AHLase 可减轻鼠伤寒沙门氏菌引起的肉鸡生长迟缓和肠道破坏，这可能与观察到的肠道微生物群失调的恢复有关。在线版本包含可在 10.1186/s40104-022-00801-4 获取的补充材料。
Salmonella Typhimurium challenge causes a huge detriment to chicken production. N-acyl homoserine lactonase (AHLase), a quorum quenching enzyme, potentially inhibits the growth and virulence of Gram-negative bacteria. However, it is unknown whether AHLase can protect chickens against S. Typhimurium challenge. This study aimed to evaluate the effects of AHLase on growth performance and intestinal health in broilers challenged by S. Typhimurium. A total of 240 one-day-old female crossbred broilers (817C) were randomly divided into 5 groups (6 replicates/group): negative control (NC), positive control (PC), and PC group supplemented with 5, 10 or 20 U/g AHLase. All birds except those in NC were challenged with S. Typhimurium from 7 to 9 days of age. All parameters related to growth and intestinal health were determined on d 10 and 14. The reductions (P < 0.05) in body weight (BW) and average daily gain (ADG) in challenged birds were alleviated by AHLase addition especially at 10 U/g. Thus, samples from NC, PC and PC plus 10 U/g AHLase group were selected for further analysis. S. Typhimurium challenge impaired (P < 0.05) intestinal morphology, elevated (P < 0.05) ileal inflammatory cytokines (IL-1β and IL-8) expression, and increased (P < 0.05) serum diamine oxidase (DAO) activity on d 10. However, AHLase addition normalized these changes. Gut microbiota analysis on d 10 showed that AHLase reversed the reductions (P < 0.05) in several beneficial bacteria (e.g. Bacilli, Bacillales and Lactobacillales), along with increases (P < 0.05) in certain harmful bacteria (e.g. Proteobacteria, Gammaproteobacteria, Enterobacteriaceae and Escherichia/Shigella) in PC group. Furthermore, AHLase-induced increased beneficial bacteria and decreased harmful bacteria were basically negatively correlated (P < 0.05) with the reductions of ileal IL-1β and IL-8 expression and serum DAO activity, but positively correlated (P < 0.05) with the increased BW and ADG. Functional prediction revealed that AHLase abolished S. Typhimurium-induced upregulations (P < 0.05) of certain pathogenicity-related pathways such as lipopolysaccharide biosynthesis, shigellosis, bacterial invasion of epithelial cells and pathogenic Escherichia coli infection of gut microbiota. Supplemental AHLase attenuated S. Typhimurium-induced growth retardation and intestinal disruption in broilers, which could be associated with the observed recovery of gut microbiota dysbiosis. The online version contains supplementary material available at 10.1186/s40104-022-00801-4.
登录
查看更多内容
影响因子：
5.2
作者：
Ilinskaya ON;Ulyanova VV;Yarullina DR;Gataullin IG
通讯作者：
Gataullin IG
DOI：
10.1084/jem.20112408
发表时间：
2012-05-07
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Jenq RR;Ubeda C;Taur Y;Menezes CC;Khanin R;Dudakov JA;Liu C;West ML;Singer NV;Equinda MJ;Gobourne A;Lipuma L;Young LF;Smith OM;Ghosh A;Hanash AM;Goldberg JD;Aoyama K;Blazar BR;Pamer EG;van den Brink MR
通讯作者：
van den Brink MR
影响因子：
1.5
作者：
Fisinin, V. I.;Il'ina, L. A.;Egorov, I. A.
通讯作者：
Egorov, I. A.
Aii810 是一种在宏基因组中发现的新型冷适应 N-酰基高丝氨酸内酯酶，可强烈减弱铜绿假单胞菌毒力因子和生物膜形成
影响因子：
5.2
作者：
Fan X;Liang M;Wang L;Chen R;Li H;Liu X
通讯作者：
Liu X
影响因子：
4.4
作者：
Cao, Yanan;He, Suxu;Yao, Bin
通讯作者：
Yao, Bin