Serum neurofilament light chain withstands delayed freezing and repeated thawing.
Serum neurofilament light chain withstands delayed freezing and repeated thawing.
复制标题
血清神经丝轻链耐受延迟冷冻和反复解冻。
DOI：
10.1038/s41598-020-77098-8
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发表时间：
2020-11-17
影响因子：
4.6
通讯作者：
Bsteh G
中科院分区：
文献类型：
--
作者：
Altmann P;Leutmezer F;Zach H;Wurm R;Stattmann M;Ponleitner M;Petzold A;Zetterberg H;Berger T;Rommer P;Bsteh G
血清神经丝轻链（sNfL）及其在神经系统疾病中暴露轴突损伤的能力在血液生物标志物研究中引起了相当大的关注。因此，随着高通量检测技术的发展，迫切需要研究这种生物标志物的分析前稳定性。我们招募了20名具有常见神经系统诊断的患者和10名对照（即没有结构性神经系统疾病的患者）。我们研究了分析前变量的变化（延迟冷冻长达24小时和重复解冻/冷冻长达三个循环）是否会影响使用最先进的Simoa技术测得的sNfL浓度。应用先进的统计学方法来暴露由于不同储存和处理条件引起的sNfL浓度的任何相关变化。我们发现，sNfL浓度保持稳定时，样品在24小时内冷冻（平均绝对差异0.2 pg/ml;个体内变异低于0.1%）。重复解冻和重新冷冻多达三次也没有显著改变测量的sNfL浓度（平均绝对差0.7 pg/ml;个体内变异低于0.2%）。我们得出结论，当样品在24小时内冷冻时，可溶性sNfL浓度在4 - 8 ° C下不受影响，并且单个等分试样可以使用多达三次。在规划未来研究时应考虑这些观察结果。
Serum neurofilament light chain (sNfL) and its ability to expose axonal damage in neurologic disorders have solicited a considerable amount of attention in blood biomarker research. Hence, with the proliferation of high-throughput assay technology, there is an imminent need to study the pre-analytical stability of this biomarker. We recruited 20 patients with common neurological diagnoses and 10 controls (i.e. patients without structural neurological disease). We investigated whether a variation in pre-analytical variables (delayed freezing up to 24 h and repeated thawing/freezing for up to three cycles) affects the measured sNfL concentrations using state of the art Simoa technology. Advanced statistical methods were applied to expose any relevant changes in sNfL concentration due to different storing and processing conditions. We found that sNfL concentrations remained stable when samples were frozen within 24 h (mean absolute difference 0.2 pg/ml; intraindividual variation below 0.1%). Repeated thawing and re-freezing up to three times did not change measured sNfL concentration significantly, either (mean absolute difference 0.7 pg/ml; intraindividual variation below 0.2%). We conclude that the soluble sNfL concentration is unaffected at 4–8 °C when samples are frozen within 24 h and single aliquots can be used up to three times. These observations should be considered for planning future studies.
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DOI：
10.1177/2055217319885987
发表时间：
2019-10-01
影响因子：
2.8
作者：
Bsteh, Gabriel;Berek, Klaus;Berger, Thomas
通讯作者：
Berger, Thomas
影响因子：
1.6
作者：
Haslacher, Helmuth;Gerner, Marlene;Perkmann, Thomas
通讯作者：
Perkmann, Thomas
影响因子：
11
作者：
Sejbaek, Tobias;Nielsen, Helle Hvilsted;Illes, Zsolt
通讯作者：
Illes, Zsolt
影响因子：
6
作者：
Mariotto, Sara;Gajofatto, Alberto;Ferrari, Sergio
通讯作者：
Ferrari, Sergio
影响因子：
6.7
作者：
KRAMER, MS;FEINSTEIN, AR
通讯作者：
FEINSTEIN, AR