Characterization of engineered tissue construct mechanical function by magnetic resonance imaging.
Characterization of engineered tissue construct mechanical function by magnetic resonance imaging.
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DOI：
10.1002/term.188
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发表时间：
2009-08
影响因子：
3.3
通讯作者：
Reddi, A. H.
中科院分区：
文献类型：
--
作者：
Neu, C. P.;Arastu, H. F.;Curtiss, S.;Reddi, A. H.
关键词：
无创磁共振成像 (MRI) 是一种能够表征活组织和工程组织中多种物理现象的技术。工程组织的机械功能是许多生物组织（例如关节软骨、脊柱和心脏）成功再生的主要终点。在这里，我们展示了 MRI 的应用来表征工程组织的机械功能。事实证明，基于相差的方法可以使用关节缺陷模型作为研究系统来表征整个天然组织和工程组织内部的详细变形场。 MRI 技术显示应变场随空间位置的不同而变化不均匀。与周围的天然软骨相比，组织结构中的应变最高。组织表面几何形状对应于在表面附近的组织内部观察到的应变场。通过钆增强成像确定，应变场进一步评估了糖胺聚糖（[GAG]）浓度的空间变化，糖胺聚糖是软骨细胞外基质中的关键蛋白聚糖。 [GAG] 浓度也根据空间位置而变化不均匀，并且与周围软骨相比，组织构建体中的浓度最低。使用多种 MRI 技术评估组织机械功能提供了补充数据，并表明变形与组织几何形状、潜在的细胞外基质成分以及所研究的模型系统中组织整合的缺乏有关。专业且先进的基于 MRI 相差的方法对于组织工程结构的机械功能的详细表征和评估非常有价值。
Noninvasive magnetic resonance imaging (MRI) is a technology that enables the characterization of multiple physical phenomena in living and engineered tissues. Mechanical function of engineered tissues is a primary endpoint for the successful regeneration of many biological tissues such as articular cartilage, spine, and heart. Here, we demonstrate the application of MRI to characterize mechanical function of engineered tissue. Phase contrast-based methods were demonstrated to characterize detailed deformation fields throughout the interior of native and engineered tissue using an articular defect model as a study system. MRI techniques revealed that strain fields varied nonuniformly depending on spatial position. Strains were highest in the tissue constructs compared to surrounding native cartilage. Tissue surface geometry corresponded to strain fields observed within the tissue interior near the surface. Strain fields were further evaluated with respect to the spatial variation in concentrations of glycosaminoglycans ([GAG]), critical proteoglycans in the extracellular matrix of cartilage, as determined by gadolinium-enhanced imaging. [GAG] concentration also varied nonuniformly depending on spatial position and was lowest in the tissue constructs compared to the surrounding cartilage. The use of multiple MRI techniques to assess tissue mechanical function provide complementary data and suggest that deformation is related to tissue geometry, underlying extracellular matrix constituents, and the lack of tissue integration in the model system studied. Specialized and advance MRI phase contrast-based methods are valuable for the detailed characterization and evaluation of mechanical function of tissue engineered constructs.
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影响因子：
4.1
作者：
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通讯作者：
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通讯作者：
Maroudas, Alice
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作者：
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通讯作者：
Reddi, A. Hari
关节软骨变形通过位移编码成像在完整的胫骨关节中确定。
影响因子：
3.3
作者：
Chan, Deva D.;Neu, Corey P.;Hu, Maury L.
通讯作者：
Hu, Maury L.