Purified Human Synovium Mesenchymal Stem Cells as a Good Resource for Cartilage Regeneration.
Purified Human Synovium Mesenchymal Stem Cells as a Good Resource for Cartilage Regeneration.
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DOI：
10.1371/journal.pone.0129096
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Akazawa C
中科院分区：
文献类型：
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作者：
Ogata Y;Mabuchi Y;Yoshida M;Suto EG;Suzuki N;Muneta T;Sekiya I;Akazawa C
骨髓间充质干细胞（Mesenchymal stem cells，MSCs）具有分化为多种细胞系和自我更新的能力，具有广泛的临床应用前景。然而，还没有很好地表征MSC的性质如何不同取决于它们所驻留的组织来源。我们先前报道了一种从骨髓中分离MSC的新技术，并揭示了细胞表面标志物（LNGFR和THY-1）的组合允许分离高度富集的MSC群体。在这项研究中，我们使用流式细胞术从滑膜中分离LNGFR+ THY-1 + MSC。结果显示滑膜组织含有显著更大百分比的LNGFR + THY-1 + MSC。我们检测了从同一患者分离的骨髓源性间充质干细胞（BM-MSCs）和滑膜源性间充质干细胞（SYN-MSCs）的殖民地形成和分化能力。这两种类型的间充质干细胞表现出显着的倾向，分化成特定的谱系。BM-MSC优先分化成骨，而在SYN-MSC培养中，观察到增强的成脂和成软骨分化。这些数据表明，从中分离MSC的组织应根据其预期的临床治疗应用进行定制。
Mesenchymal stem cells (MSCs) have the ability to differentiate into a variety of lineages and to renew themselves without malignant changes, and thus hold potential for many clinical applications. However, it has not been well characterized how different the properties of MSCs are depending on the tissue source in which they resided. We previously reported a novel technique for the prospective MSC isolation from bone marrow, and revealed that a combination of cell surface markers (LNGFR and THY-1) allows the isolation of highly enriched MSC populations. In this study, we isolated LNGFR+ THY-1 + MSCs from synovium using flow cytometry. The results show that the synovium tissue contained a significantly larger percentage of LNGFR + THY-1 + MSCs. We examined the colony formation and differentiation abilities of bone marrow-derived MSCs (BM-MSCs) and synovium-derived MSCs (SYN-MSCs) isolated from the same patients. Both types of MSCs exhibited a marked propensity to differentiate into specific lineages. BM-MSCs were preferentially differentiated into bone, while in the SYN-MSC culture, enhanced adipogenic and chondrogenic differentiation was observed. These data suggest that the tissue from which MSCs are isolated should be tailored according to their intended clinical therapeutic application.
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小鼠骨髓中多能间充质干细胞的前瞻性鉴定、分离和系统移植。
DOI：
10.1084/jem.20091046
发表时间：
2009-10-26
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Morikawa S;Mabuchi Y;Kubota Y;Nagai Y;Niibe K;Hiratsu E;Suzuki S;Miyauchi-Hara C;Nagoshi N;Sunabori T;Shimmura S;Miyawaki A;Nakagawa T;Suda T;Okano H;Matsuzaki Y
通讯作者：
Matsuzaki Y
影响因子：
5.9
作者：
Mabuchi, Yo;Morikawa, Satoru;Harada, Seiko;Niibe, Kunimichi;Suzuki, Sadafumi;Renault-Mihara, Francois;Houlihan, Diarmaid D.;Akazawa, Chihiro;Okano, Hideyuki;Matsuzaki, Yumi
通讯作者：
Matsuzaki, Yumi
影响因子：
2.8
作者：
Matsukura, Yu;Muneta, Takeshi;Tsuji, Kunikazu;Miyatake, Kazumasa;Yamada, Jun;Abula, Kahaer;Koga, Hideyuki;Tomita, Makoto;Sekiya, Ichiro
通讯作者：
Sekiya, Ichiro
影响因子：
4.5
作者：
Hanley PJ;Mei Z;da Graca Cabreira-Hansen M;Klis M;Li W;Zhao Y;Durett AG;Zheng X;Wang Y;Gee AP;Horwitz EM
通讯作者：
Horwitz EM
影响因子：
64.8
作者：
通讯作者：
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