Mouse synovial mesenchymal stem cells increase in yield with knee inflammation.
Mouse synovial mesenchymal stem cells increase in yield with knee inflammation.
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DOI：
10.1002/jor.22753
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发表时间：
2015-02
影响因子：
2.8
通讯作者：
Sekiya, Ichiro
中科院分区：
文献类型：
--
作者：
Matsukura, Yu;Muneta, Takeshi;Tsuji, Kunikazu;Miyatake, Kazumasa;Yamada, Jun;Abula, Kahaer;Koga, Hideyuki;Tomita, Makoto;Sekiya, Ichiro
尽管小鼠研究具有各种优势，但在小鼠中收获足够数量的滑膜间充质干细胞（MSC）是困难的。我们研究了小鼠滑膜炎症中MSC的总产量是否增加。在关节内注射角叉菜胶后3、7和14天，从5只小鼠的10个膝关节收集髌前脂肪垫（IFP）。还从未处理的膝关节收获10个IFP作为对照。在初始铺板后7天，比较4组（n = 4-6）之间的细胞总产量。分析收获的细胞的多潜能性和表面表位。并对4组间膝关节滑膜炎进行组织学比较。第3天和第7天处理组的细胞数量显著高于其他组。收获的细胞具有MSCs的特征。3天和7天治疗组的滑膜炎明显比其他组严重。滑膜炎评分与IFP细胞总产量之间似乎存在相关性。在小鼠中，通过诱导炎症可以将产量提高50倍。这种方法使得在小鼠模型中分析滑膜MSC软骨再生的分子机制成为可能。© 2014作者。© 2014作者。由Wiley Periodicals，Inc.出版的骨科研究杂志。我代表矫形外科研究协会J Orthop Res 33：246-253，2015.
Even though mouse studies have various advantages, harvesting an adequate number of synovial mesenchymal stem cells (MSCs) is difficult in mice. We investigated whether the total yield of MSCs increased in synovium with inflammation in mice. Infrapatellar fat pads (IFPs) were harvested from 10 knees of 5 mice 3, 7, and 14 days after intraarticular injection of carrageenan. Ten IFPs were also harvested from untreated knees as a control. Seven days after initial plating, the total yield of cells was compared among the 4 groups (n = 4–6). The harvested cells were analyzed for multipotentiality and surface epitopes. Furthermore, knee synovitis was compared among the 4 groups in histology. The number of cells in the 3 and 7 days treated group was significantly higher than the other groups. The harvested cells had characteristics of MSCs. Synovitis in the 3 and 7 days treated groups was significantly severer than the other groups. There seemed to be a relationship between the synovitis score and the total yield of cells derived from IFPs. In mice, it became possible to increase the yield 50-fold by inducing inflammation. This method makes it possible to analyze the molecular mechanisms of cartilage regeneration of synovial MSCs in mice models. © 2014 The Authors. © 2014 The Authors. Journal of Orthopaedic Research published by Wiley Periodicals, Inc. on behalf of the Orthopaedic Research Society. J Orthop Res 33:246–253, 2015.
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影响因子：
4.2
作者：
Matsukura Y;Muneta T;Tsuji K;Koga H;Sekiya I
通讯作者：
Sekiya I
影响因子：
7.2
作者：
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通讯作者：
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影响因子：
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作者：
Mochizuki, T;Muneta, T;Sekiya, I
通讯作者：
Sekiya, I
影响因子：
4.9
作者：
Bang JS;Oh DH;Choi HM;Sur BJ;Lim SJ;Kim JY;Yang HI;Yoo MC;Hahm DH;Kim KS
通讯作者：
Kim KS
影响因子：
2.9
作者：
Asakura, A;Komaki, M;Rudnicki, MA
通讯作者：
Rudnicki, MA