Fifteen days of microgravity causes growth in calvaria of mice.
Fifteen days of microgravity causes growth in calvaria of mice.
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DOI：
10.1016/j.bone.2013.06.009
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发表时间：
2013-10
期刊：
影响因子：
4.1
通讯作者：
Hargens, Alan R.
中科院分区：
文献类型：
--
作者：
Zhang, Bing;Cory, Esther;Bhattacharya, Roshmi;Sah, Robert;Hargens, Alan R.
骨重建可能发生在航天作为骨骼卸载和头部液体转移的反应。虽然卸载会导致暴露于微重力的动物腿部骨量和密度的显著损失，但伴随微重力引起的液体再分布的血液和间质液流量增加可能会在头部引起相反的影响。在STS-131使命中，随机选择7只C57 BL/6小鼠暴露于15天的微重力环境，而8只同窝出生的小鼠作为地面对照。使命完成后，在微型计算机断层扫描仪上对所有15只小鼠的颅骨进行成像。将标准化的矩形体积放置在每个颅骨的顶骨上进行分析，并确定三个参数来测量顶骨体积的增加：骨体积（BV），横截面厚度（CsTh）和组织矿物质密度（TMD）。与地面对照组相比，微重力暴露导致空间飞行组的BV在统计学上显著增加-空间飞行组的平均值±标准差为10.48 ± 0.47%，而地面对照组为9.64 ± 0.79%（p<0.05）。CsTh表现出从GC组的0.099 ±0.006 mm增加至SF组的0.104 ±0.005 mm的趋势（p=0.12）。两组的TMD相似，GC为0.878 ±0.029 g/cc，SF为0.893 ± 0.028 g/cc（p=0.31）。我们的研究结果表明，微重力会导致通常不承重的颅骨发生适应性变化。这些发现表明，流体转移单独伴随微重力可能会启动骨重建独立的骨骼组织负荷。
Bone remodeling may occur in spaceflight as a response to skeletal unloading and head-ward fluid shifts. While unloading causes significant loss of bone mass and density in legs of animals exposed to microgravity, increased blood and interstitial fluid flows accompanying microgravity-induced fluid redistribution may elicit an opposite effect in the head. Seven C57BL/6 mice were randomly chosen for exposure to 15 days of microgravity on the STS-131 mission, while eight littermates served as ground controls. Upon mission completion, all 15 mice calvariae were imaged on a micro-computed tomography scanner. A standardized rectangular volume was placed on the parietal bones of each calvaria for analyses, and three parameters were determined to measure increased parietal bone volume: bone volume (BV), cross-sectional thickness (CsTh), and tissue mineral density (TMD). Microgravity exposure caused a statistically significant increase in BV of the space flight (SF) group compared to the ground control (GC) group – the mean ± SD for SF group was 10.48 ± 0.47 %, compared to 9.64 ± 0.79 % for GC group (p<0.05). CsTh demonstrated a trend of increase from 0.099 ±0.006 mm in the GC group to 0.104 ±0.005 mm in the SF group (p=0.12). TMD was similar between the two groups with 0.878 ±0.029 g/cc for GC and 0.893 ± 0.028 g/cc for SF (p=0.31). Our results indicate that microgravity causes adaptive changes in calvarial bones that do not normally bear weight. These findings suggest that fluid shifts alone accompanying microgravity may initiate bone remodeling independent of skeletal loading by tissue.
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