Growth inhibitory, bactericidal, and morphostructural effects of dehydrocostus lactone from Magnolia sieboldii Leaves on antibiotic-susceptible and -resistant strains of Helicobacter pylori.
Growth inhibitory, bactericidal, and morphostructural effects of dehydrocostus lactone from Magnolia sieboldii Leaves on antibiotic-susceptible and -resistant strains of Helicobacter pylori.
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DOI：
10.1371/journal.pone.0095530
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ahn YJ
中科院分区：
文献类型：
--
作者：
Lee HK;Song HE;Lee HB;Kim CS;Koketsu M;Ngan LT;Ahn YJ
幽门螺杆菌与多种上胃肠道疾病有关，如胃炎症、十二指肠溃疡和胃溃疡。本研究的目的是评估抗H。与商业纯DCL、两种先前已知的倍半萜内酯（木香内酯和银胶菊内酯）、（-）-表没食子儿茶素没食子酸酯和四种抗生素相比，来自天女木兰叶的倍半萜内酯脱氢木香内酯（DCL）对幽门螺杆菌的作用。研究了天然DCL对大肠杆菌的抗菌活性。pylori ATCC 700392和H. pylori ATCC 700824菌株（MIC，4.9和4.4mg/L）与商业DCL相似，并且比costuntamine、parthenetamine和EGCG更有效。DCL的活性略低于甲硝唑（MIC分别为1.10和1.07mg/L）。DCL对敏感株和耐药株的抗菌活性基本相同，对阿莫西林（PED 503G株MIC为11.1mg/L）、克拉霉素（PED 3582GA株MIC为49.8mg/L）、甲硝唑（PED 3582GA株MIC为21.6mg/L）和甲硝唑（PED 3582GA株MIC为11.1mg/L）的耐药率分别为99.1%和99.1%。pylori ATCC 43504菌株; 71.1 mg/L，221菌株）或四环素（14.2 mg/L，B菌株）。这一发现表明DCL和抗生素没有共同的作用模式。DCL对H. pylori ATCC 43504不受检查的pH值（4.0 - 7.0）的影响。DCL引起相当大的转化为球形体（在8和4 mg/L的DCL下48 h为94 vs 49%）。Westernblot分析表明，H. pylori ATCC 43504的UreA单体条带不受10 mM DCL的影响，而UreA单体条带在0.1 mM（-）-表没食子儿茶素没食子酸酯时完全消失。降低抗生素水平的全球努力证明了对M.含有DCL的天女木兰叶衍生材料可作为潜在的抗菌产品或预防或根除耐药H.幽门。
Helicobacter pylori is associated with various diseases of the upper gastrointestinal tract, such as gastric inflammation and duodenal and gastric ulcers. The aim of the study was to assess anti-H. pylori effects of the sesquiterpene lactone dehydrocostus lactone (DCL) from Magnolia sieboldii leaves, compared to commercial pure DCL, two previously known sesquiterpene lactones (costunolide and parthenolide), (–)-epigallocatechin gallate, and four antibiotics. The antibacterial activity of natural DCL toward antibiotic-susceptible H. pylori ATCC 700392 and H. pylori ATCC 700824 strains (MIC, 4.9 and 4.4 mg/L) was similar to that of commercial DCL and was more effective than costunolide, parthenolide, and EGCG. The activity of DCL was slightly lower than that of metronidazole (MIC, 1.10 and 1.07 mg/L). The antibacterial activity of DCL was virtually identical toward susceptible and resistant strains, even though resistance to amoxicillin (MIC, 11.1 mg/L for PED 503G strain), clarithromycin (49.8 mg/L for PED 3582GA strain), metronidazole (21.6 mg/L for H. pylori ATCC 43504 strain; 71.1 mg/L for 221 strain), or tetracycline (14.2 mg/L for B strain) was observed. This finding indicates that DCL and the antibiotics do not share a common mode of action. The bactericidal activity of DCL toward H. pylori ATCC 43504 was not affected by pH values examined (4.0–7.0). DCL caused considerable conversion to coccoid form (94 versus 49% at 8 and 4 mg/L of DCL for 48 h). The Western blot analysis revealed that urease subunits (UreA and UreB) of H. pylori ATCC 43504 were not affected by 10 mM of DCL, whereas UreA monomer band completely disappeared at 0.1 mM of (–)-epigallocatechin gallate. Global efforts to reduce the level of antibiotics justify further studies on M. sieboldii leaf-derived materials containing DCL as potential antibacterial products or a lead molecule for the prevention or eradication of drug-resistant H. pylori.
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作者：
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