Green tea catechin, epigallocatechin-3-gallate, attenuates the cell viability of human non-small-cell lung cancer A549 cells via reducing Bcl-xL expression.
Green tea catechin, epigallocatechin-3-gallate, attenuates the cell viability of human non-small-cell lung cancer A549 cells via reducing Bcl-xL expression.
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DOI：
10.3892/etm.2014.1719
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发表时间：
2014-07
影响因子：
2.7
通讯作者：
Motoya T
中科院分区：
文献类型：
--
作者：
Sonoda JI;Ikeda R;Baba Y;Narumi K;Kawachi A;Tomishige E;Nishihara K;Takeda Y;Yamada K;Sato K;Motoya T
临床和流行病学研究表明，饮用绿色茶对健康有许多有益的影响。表没食子儿茶素-3-没食子酸酯（EGCg）是存在于绿色茶中的主要多酚化合物，作为活性成分受到了广泛关注。在众多有前途的概况EGCg，本研究的重点是抗癌作用。EGCg诱导的细胞凋亡和随后的细胞生长抑制已在许多细胞培养研究中得到证实。然而，凋亡性细胞死亡的潜在机制仍不清楚。因此，本研究的目的是确定介导EGCg促凋亡细胞死亡的主要分子。在A549人非小细胞肺癌细胞系中评价EGCg对细胞增殖和调节凋亡性细胞死亡的mRNA的诱导的作用。此外，通过显微镜检查评估了100 μM EGCg处理24 h后A549细胞的形态学变化。MTT法显示EGCg以剂量依赖性方式（3-100 μM）显著抑制细胞增殖。用100 μM EGCg孵育24 h后，A549细胞中特大B细胞淋巴瘤（Bcl-xL）的mRNA表达水平降低。因此，结果表明，EGCg抑制细胞增殖可能是通过抑制细胞死亡抑制基因Bcl-xL的表达来实现的。
Clinical and epidemiological studies have indicated that the consumption of green tea has a number of beneficial effects on health. Epigallocatechin-3-gallate (EGCg), the major polyphenolic compound present in green tea, has received much attention as an active ingredient. Among the numerous promising profiles of EGCg, the present study focused on the anticancer effects. Apoptosis induced by EGCg and subsequent cell growth suppression have been demonstrated in a number of cell culture studies. However, the underlying mechanism of apoptotic cell death remains unclear. Thus, the aim of the present study was to identify the major molecule that mediates proapoptotic cell death by EGCg. The effect of EGCg on cell proliferation and the induction of mRNA that modulates apoptotic cell death was evaluated in the A549 human non-small-cell lung cancer cell line. In addition, morphological changes were assessed by microscopy in A549 cells that had been treated with 100 μM EGCg for 24 h. The MTT assay revealed that cell proliferation was significantly reduced by EGCg in a dose-dependent manner (3–100 μM). The mRNA expression level of B-cell lymphoma-extra large (Bcl-xL) was decreased in A549 cells following 24 h incubation with 100 μM EGCg. Therefore, the results indicated that the inhibition of cell proliferation by EGCg may be achieved via suppressing the expression of the cell death-inhibiting gene, Bcl-xL.
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DOI：
10.1080/01635580903285056
发表时间：
2009
期刊：
Nutrition and cancer
影响因子：
--
作者：
Khan N;Adhami VM;Mukhtar H
通讯作者：
Mukhtar H
影响因子：
5
作者：
Kurahashi, Norie;Sasazuki, Shizuka;Tsugane, Shoichiro
通讯作者：
Tsugane, Shoichiro
影响因子：
3.2
作者：
Yamauchi, Rieko;Sasaki, Kaori;Yoshida, Kenichi
通讯作者：
Yoshida, Kenichi
影响因子：
9.7
作者：
Paschka, AG;Butler, R;Young, CYF
通讯作者：
Young, CYF
影响因子：
5.1
作者：
Imai, K;Suga, K;Nakachi, K
通讯作者：
Nakachi, K