Arenimonas caeni sp. nov., isolated from activated sludge.

Arenimonas caeni sp. nov., isolated from activated sludge.
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镰刀菌属

DOI:
10.1099/ijsem.0.002937
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发表时间:
2018-07
影响因子:
2.8
通讯作者:
Zhu Guoping
Zhu Guoping
中科院分区:
生物学3区
文献类型:
--
作者:
Liu Hongming;Ren Lei;Lu Peng;Sun Lina;Zhu Guoping

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从安徽芜湖某城市污水处理厂的活性污泥中分离到一株革兰氏阴性、无芽胞、能游动、杆状的菌株z29T。16S rRNA基因的系统发育分析表明,菌株z29T与Arenimonas donghasis Ho3-R19T(97.14 %)、Arenimonas aestuarii S2-21T(96.46 %)、Arenimonas daejeonensis T7-07T(96.24 %)和桃源Arenimonas TN 2-31at(96.23 %)的亲缘关系最近。菌株z29T唯一的呼吸对苯二酚是泛苯二酚8(Q-8)。主要的细胞脂肪酸(>10 %)是iso-C15 : 0、iso-C16 : 0和总和特征9(iso-C17 : 1ω9c和/或C16 : 010-m)。主要的极性脂为二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和一种单一的fi磷脂。基因组DNAG+C含量为70.2 摩尔%。Z29T菌株与东海沙门氏菌H03-R19T的基因组同源性为83.72 %。根据表型和化学分类结果,结合系统发育分析,Z29T菌株被归类为Arenimonas属的一个新种,命名为Arenimonas caeni。11月是提议的。模式菌株为Z29T(=JCM 32091T=CCTCC AB 2017067T)。
A Gram-stain-negative, non-spore-forming, motile and rod-shaped strain, z29T, was isolated from the active sludge of a municipal wastewater treatment plant at Wuhu, Anhui, PR China. Phylogenetic analysis of the 16S rRNA gene revealed that strain z29T is most closely related to the genus Arenimonas, showing the highest similarity to Arenimonas donghaensis HO3-R19T (97.14 %), Arenimonas aestuarii S2-21T (96.46 %), Arenimonas daejeonensis T7-07T (96.24 %) and Arenimonas taoyuanensis YN2-31AT (96.23 %). The only respiratory quinone of strain z29T was ubiquinone 8 (Q-8). The major cellular fatty acids (>10 %) were iso-C15 : 0, iso-C16 : 0 and summed feature 9 (iso-C17 : 1ω9c and/or C16 : 010-methyl). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The genomic DNA G+C content was 70.2 mol%. Genomic comparison between strain z29T and Arenimonas donghaensis HO3-R19T revealed 83.72 % average nucleotide identity. Based on the phenotypic and chemotaxonomic results together with phylogenetical analysis, strain z29T is classified as representing a novel species of the genus Arenimonas, for which the name Arenimonas caeni sp. nov. is proposed. The type strain is z29T (=JCM 32091T=CCTCC AB 2017067T).
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