Cell proliferation and survival in the mating circuit of adult male hamsters: effects of testosterone and sexual behavior.
Cell proliferation and survival in the mating circuit of adult male hamsters: effects of testosterone and sexual behavior.
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DOI：
10.1016/j.yhbeh.2008.08.001
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发表时间：
2008-11
影响因子：
3.5
通讯作者：
Wood RI
中科院分区：
文献类型：
--
作者：
Antzoulatos E;Magorien JE;Wood RI
性腺类固醇对成人大脑的短暂作用促进了社会行为，包括生殖。在雄性啮齿动物中，睾酮作用于后内侧杏仁核（MeP）和内侧视前区（MPOA）以促进交配。成人神经发生发生在这两个区域。目前的研究确定了睾酮和/或性行为是否促进MeP和MPOA中的细胞增殖和存活。使用胸苷类似物BrdU进行两个实验。首先，在BrdU后24小时或7周比较性腺完整和去势的雄性仓鼠（n=6/组）。在MeP中，BrdU后24小时，睾酮刺激细胞增殖（完整：22.8±3.9个细胞/mm 2，去势：13.2± 1.4个细胞/mm 2）。睾酮不促进MPOA中的细胞增殖。BrdU后7周，两个区域的细胞存活率均较低（MeP：2.5±0.6和MPOA：1.7±0.2个细胞/mm 2），睾酮未增强。在实验2中，性腺完整的性经验丰富的动物每周交配，以确定是否定期的神经激活提高细胞存活7周后BrdU在MeP和MPOA。每周交配未能增加MeP（8.1±1.6 vs. 9.9±3.2 cells/mm 2）或MPOA（3.9±0.7 vs. 3.4±0.3 cells/mm 2）中的细胞存活率。此外，注射BrdU时的交配不会刺激MeP（8.9±1.7 vs. 8.1±1.6 cells/mm 2）或MPOA（3.6±0.5 vs. 3.9±0.7 cells/mm 2）中的细胞增殖。总之，我们的研究结果表明，在交配电路的神经发生的能力有限。具体而言，MeP和MPOA中的细胞增殖受到睾酮的不同影响，并且生殖活动不会增强两个区域中新细胞的出生和存活。
The transient actions of gonadal steroids on the adult brain facilitate social behaviors, including reproduction. In male rodents, testosterone acts in the posterior medial amygdala (MeP) and medial preoptic area (MPOA) to promote mating. Adult neurogenesis occurs in both regions. The current study determined if testosterone and/or sexual behavior promote cell proliferation and survival in MeP and MPOA. Two experiments were conducted using the thymidine analog BrdU. First, gonad-intact and castrated male hamsters (n=6/group) were compared 24 hours or 7 weeks after BrdU. In MeP, testosterone stimulated cell proliferation 24 hours after BrdU (intact: 22.8±3.9 cells/mm2, castrate: 13.2±1.4cells/mm2). Testosterone did not promote cell proliferation in MPOA. Seven weeks after BrdU, cell survival was sparse in both regions (MeP: 2.5±0.6 and MPOA: 1.7±0.2 cells/mm2), and was not enhanced by testosterone. In Experiment 2, gonad-intact sexually-experienced animals were mated weekly to determine if regular neural activation enhances cell survival 7 weeks after BrdU in MeP and MPOA. Weekly mating failed to increase cell survival in MeP (8.1±1.6 vs. 9.9±3.2 cells/mm2) or MPOA (3.9±0.7 vs. 3.4±0.3 cells/mm2). Furthermore, mating at the time of BrdU injection did not stimulate cell proliferation in MeP (8.9±1.7 vs. 8.1±1.6 cells/mm2) or MPOA (3.6±0.5 vs. 3.9±0.7 cells/mm2). Taken together, our results demonstrate a limited capacity for neurogenesis in the mating circuitry. Specifically, cell proliferation in MeP and MPOA are differentially influenced by testosterone, and the birth and survival of new cells in either region are not enhanced by reproductive activity.
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DOI：
10.1002/neu.10273
发表时间：
2003-12-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Fowler, CD;Freeman, ME;Wang, ZX
通讯作者：
Wang, ZX
影响因子：
2.9
作者：
LEHMAN, MN;WINANS, SS
通讯作者：
WINANS, SS
DOI：
10.1016/s0163-1047(80)91503-4
发表时间：
1980-01-01
期刊：
BEHAVIORAL AND NEURAL BIOLOGY
影响因子：
--
作者：
LISK, RD;HEIMANN, J
通讯作者：
HEIMANN, J
影响因子：
2.9
作者：
MIERNICKI, M;POSPICHAL, MW;POWERS, JB
通讯作者：
POWERS, JB
影响因子：
10.6
作者：
Gould, E;Tanapat, P;Hastings, N
通讯作者：
Hastings, N