The Surge of Hypervirulent ST398 MRSA Lineage With Higher Biofilm-Forming Ability Is a Critical Threat to Clinics.
The Surge of Hypervirulent ST398 MRSA Lineage With Higher Biofilm-Forming Ability Is a Critical Threat to Clinics.
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具有较高生物膜形成能力的高毒力 ST398 MRSA 谱系的激增对诊所构成严重威胁
DOI：
10.3389/fmicb.2021.636788
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发表时间：
2021
影响因子：
5.2
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Lu H;Zhao L;Si Y;Jian Y;Wang Y;Li T;Dai Y;Huang Q;Ma X;He L;Li M
耐甲氧西林金黄色葡萄球菌（MRSA）社区相关（CA）感染的全球增加是一个主要的医疗保健问题。虽然序列型（ST）398 MRSA最初被描述为牲畜相关（LA）谱系，但在我们之前的研究和其他后续研究中，随后在中国报道了不与牲畜接触的人适应MRSA（HO-MRSA）ST 398。近年来，ST 398 HO-MRSA在中国的比例也显著增加。基于3878 S.根据2008年至2018年在综合医院收集的金黄色葡萄球菌分离株，我们鉴定了56株ST 398 HO-MRSA分离株。我们在前期的研究中对其中4个早期出现的分离株进行了全基因组测序。在这里，通过使用WGS对后出现的52株分离株和分析谱系的系统发育动力学，我们发现，50株与前4株菌株聚在一起，使其成为MSSA克隆和其他MRSA克隆的主要分支，尽管ST 398 HO-MRSA具有多个起源。基于WGS数据的耐药性和毒力基因分析表明，ST 398 HO-MRSA主进化枝在两个部分中表现出相似的模式。此外，它们都携带一个保守的原噬菌体3的变体，以保证毒力和一个短的SCCmec D类V型元件，以保持相当低的甲氧西林耐药性。进一步的表型研究证实，流行性HO-MRSA ST 398在保持高毒力的同时显示出增强的生物膜形成能力。HO-MRSA ST 398亚型的毒力和生物膜形成的双重优势促进了其在社区甚至医疗环境中的适应性，这对临床S.金黄色葡萄球菌感染。因此，需要进一步监测，以预防和控制HO-MRSA ST 398在未来对公共卫生的影响。
The global increase of community-associated (CA) infections with methicillin-resistant Staphylococcus aureus (MRSA) is a major healthcare problem. Although sequence type (ST) 398 MRSA was first described as a livestock-associated (LA) lineage, human-adapted MRSA (HO-MRSA) ST398 without livestock contact has subsequently been reported from China in our previous study and other later research. The proportion of ST398 HO-MRSA has also remarkably increased in recent years in China. Based on 3878 S. aureus isolates that were collected in a general hospital between 2008 and 2018, we identified 56 ST398 HO-MRSA isolates. The four early appearing isolates of them have been sequenced by whole-genome sequencing (WGS) in our previous study. Here, by usage of WGS on the later-appearing 52 isolates and analyzing the phylogenetic dynamics of the linage, we found that 50 isolates clustered together with the former 4 isolates, making it a main clade out of MSSA clones and other MRSA clones, although ST398 HO-MRSA evolved with multiple origins. Drug resistance and virulence gene analysis based on the WGS data demonstrated that ST398 HO-MRSA main clade exhibited a similar pattern in both parts. Furthermore, they all carried a conserved variant of prophage 3 to guarantee virulence and a short SCCmec type V element of class D to maintain considerable lower methicillin resistance. Further phenotypical research verified that the epidemic HO-MRSA ST398 displayed enhanced biofilm formation ability when keeping high virulence. The dual advantages of virulence and biofilm formation in the HO-MRSA ST398 subtype promote their fitness in the community and even in the healthcare environment, which poses a serious threat in clinical S. aureus infections. Therefore, further surveillance is required to prevent and control the problematic public health impact of HO-MRSA ST398 in the future.
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影响因子：
6.4
作者：
Price LB;Stegger M;Hasman H;Aziz M;Larsen J;Andersen PS;Pearson T;Waters AE;Foster JT;Schupp J;Gillece J;Driebe E;Liu CM;Springer B;Zdovc I;Battisti A;Franco A;Zmudzki J;Schwarz S;Butaye P;Jouy E;Pomba C;Porrero MC;Ruimy R;Smith TC;Robinson DA;Weese JS;Arriola CS;Yu F;Laurent F;Keim P;Skov R;Aarestrup FM
通讯作者：
Aarestrup FM
影响因子：
11
作者：
Huang, Yhu-Chering;Chen, Chih-Jung
通讯作者：
Chen, Chih-Jung
DOI：
10.1093/infdis/jir845
发表时间：
2012-03-01
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Rudkin JK;Edwards AM;Bowden MG;Brown EL;Pozzi C;Waters EM;Chan WC;Williams P;O'Gara JP;Massey RC
通讯作者：
Massey RC
耐甲氧西林金黄色葡萄球菌 (MRSA) 感染的减少归因于主要 MRSA ST239 克隆的消失，上海，2008-2017 年。
影响因子：
13.2
作者：
Dai, Yingxin;Liu, Junlan;Li, Min
通讯作者：
Li, Min
影响因子：
3.3
作者：
Khanna, T.;Friendship, R.;Weese, J. S.
通讯作者：
Weese, J. S.