Effects of electrode surface modification with chlorotoxin on patterning single glioma cells.
Effects of electrode surface modification with chlorotoxin on patterning single glioma cells.
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DOI：
10.1039/c0cp02908d
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发表时间：
2011-05-21
期刊：
影响因子：
--
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Asphahani F;Zheng X;Veiseh O;Thein M;Xu J;Ohuchi F;Zhang M
由单个癌细胞阵列构图的微芯片可以成为研究肿瘤生物学、医学诊断和药物筛选的有效平台。然而，在微阵列的特定位置构图和保留可存活的单个癌细胞可能是具有挑战性的。在这项研究中，我们使用一种肿瘤细胞特异性的多肽，氯毒素(CTX)，来介导胶质瘤细胞在金微电极阵列上的吸附，并研究了三种表面修饰方案对单细胞构型的影响，包括物理吸附，N-羟基琥珀酰亚胺(NHS)介导的共价键，以及通过丁二酰亚胺碘乙酸酯和Traut试剂交联的共价键(SIA-Traut)。高分辨X射线光电子能谱证实了CTX在微电极上的固定化。与共价结合的CTX相比，物理吸附的CTX对细胞黏附显示出更好的支持，并且在将黏附的细胞限制在电极上时更有效。此外，通过阻抗测量实时量化细胞在微电极上的黏附和扩散，这表明来自物理吸附的CTX电极的阻抗信号比来自共价结合的CTX电极的信号大四倍。
A microchip patterned with arrays of single cancer cells can be an effective platform for study of tumor biology, medical diagnostics, and drug screening. However, patterning and retaining viable single cancer cells on defined sites of the microarray can be challenging. In this study we used a tumor cell-specific peptide, chlorotoxin (CTX), to mediate glioma cell ahdesion on arrays of gold microelectrodes and investigated the effects of three surface modification schemes for conjugation of CTX to the microelectrodes on single cell patterning, which include physical adsorption, covalent bonding mediated by N-hydroxysuccinimide (NHS), and covalent bonding via crosslinking succinimidyl iodoacetate and Traut’s (SIA-Traut) regents. The CTX immobilization to microelectrodes was confirmed by high-resolution X-ray photoelectron spectroscopy. Physically adsorbed CTX showed better support for cell adheison and is more effective in confining adhered cells on the electrodes than covalently-bound CTX. Furthermore, cell adhesion and spreading on microelectrodes were quantified in real-time by impedance measurements, which revealed an impedance signal from physically adsorbed CTX electrodes four times greater than the signal from covalently-bound CTX electrodes.
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