Different Subgroups of Cholinergic Neurons in the Basal Forebrain Are Distinctly Innervated by the Olfactory Regions and Activated Differentially in Olfactory Memory Retrieval.
Different Subgroups of Cholinergic Neurons in the Basal Forebrain Are Distinctly Innervated by the Olfactory Regions and Activated Differentially in Olfactory Memory Retrieval.
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基底前脑胆碱能神经元的不同亚群明显受嗅觉区域支配，并在嗅觉记忆检索中被不同地激活
DOI：
10.3389/fncir.2018.00099
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发表时间：
2018
影响因子：
3.5
通讯作者：
Xu F
中科院分区：
文献类型：
--
作者：
Zheng Y;Feng S;Zhu X;Jiang W;Wen P;Ye F;Rao X;Jin S;He X;Xu F
哺乳动物的基底前脑(BF)是一种向皮质和边缘结构提供初级胆碱能输入的异质结构，在学习/记忆和注意等各种生理过程中发挥着至关重要的作用。尽管BF胆碱能神经元(BFCNs)参与了与嗅觉相关的记忆，但其潜在的神经回路仍然知之甚少。在这里，我们结合病毒跨突触示踪系统和Chat-cre转基因小鼠来系统地揭示嗅觉系统与BFCNs不同亚群之间的关系。逆行腺相关病毒和狂犬病病毒(AAV-RV)示踪表明，不同亚区的BFCNs从多个嗅皮层接受不同的输入。内侧和尾侧水平斜角带(HDB)、大细胞视前区(MCPO)和腹侧无名质(SI)的胆碱能神经元主要接受嗅球(OB)、前嗅核(AON)、内嗅皮层(ENT)、杏仁基底外侧核(PC)、梨状皮质(PC)和海马(HIP)的传入，而内侧隔(MS/DB)和头侧HDB的一部分(以下简称MS/DB复合体，MS/DBC)主要来自HIP；和基底Meynert核(NBM)和背侧SI(以下简称NBM复合体)，主要来自中央杏仁核。顺行性水疱性口炎病毒(VSV)示踪进一步证实了OB对BF的主要靶点是hMSC。为了将BFCNs与嗅觉功能之间的结构关系联系起来，用c-fos免疫组织化学染色方法对嗅觉相关任务中激活的BF神经元进行了定位。结果发现，在GO/NO-GO嗅觉辨别任务中，部分BFCNs被激活，但激活模式不同。有趣的是，hMSC中的BFCNs比其他亚区的BFCNs激活得更明显。因此，我们的数据表明，在BFCNs的不同亚群中，hMSC在结构和功能上都与嗅觉系统关系更密切。这项工作为不同的BFNC亚群在嗅觉相关记忆中的不同作用提供了证据。
The mammalian basal forebrain (BF), a heterogenous structure providing the primary cholinergic inputs to cortical and limbic structures, plays a crucial role in various physiological processes such as learning/memory and attention. Despite the involvement of the BF cholinergic neurons (BFCNs) in olfaction related memory has been reported, the underlying neural circuits remain poorly understood. Here, we combined viral trans-synaptic tracing systems and ChAT-cre transgenic mice to systematically reveal the relationship between the olfactory system and the different subsets of BFCNs. The retrograde adeno-associated virus and rabies virus (AAV-RV) tracing showed that different subregional BFCNs received diverse inputs from multiple olfactory cortices. The cholinergic neurons in medial and caudal horizontal diagonal band Broca (HDB), magnocellular preoptic area (MCPO) and ventral substantia innominate (SI; hereafter HMS complex, HMSc) received the inputs from the entire olfactory system such as the olfactory bulb (OB), anterior olfactory nucleus (AON), entorhinal cortex (ENT), basolateral amygdala and especially the piriform cortex (PC) and hippocampus (HIP); while medial septum (MS/DB) and a part of rostral HDB (hereafter MS/DB complex, MS/DBc), predominantly from HIP; and nucleus basalis Meynert (NBM) and dorsal SI (hereafter NBM complex, NBMc), mainly from the central amygdala. The anterograde vesicular stomatitis virus (VSV) tracing further validated that the major target of the OB to the BF is HMSc. To correlate these structural relations between the BFCNs and olfactory functions, the neurons activated in the BF during olfaction related task were mapped with c-fos immunostaining. It was found that some of the BFCNs were activated in go/no-go olfactory discrimination task, but with different activated patterns. Interestingly, the BFCNs in HMSc were more significantly activated than the other subregions. Therefore, our data have demonstrated that among the different subgroups of BFCNs, HMSc is more closely related to the olfactory system, both structurally and functionally. This work provides the evidence for distinct roles of different subsets of BFNCs in olfaction associated memory.
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影响因子：
3.5
作者：
Harrison TC;Pinto L;Brock JR;Dan Y
通讯作者：
Dan Y
影响因子：
2.9
作者：
Martin, Megan M.;Winter, Shawn S.;Cheatwood, Joseph L.;Carter, Lynniece A.;Jones, Jeana L.;Weathered, Scott L.;Wagner, Steven J.;Wallace, Douglas G.
通讯作者：
Wallace, Douglas G.
影响因子：
3.5
作者：
Knox D;Keller SM
通讯作者：
Keller SM
影响因子：
5.3
作者：
Ma, Ming;Luo, Minmin
通讯作者：
Luo, Minmin
胆碱能系统全脑图谱的生成和基底前脑胆碱能神经元的介观投影组分析。
DOI：
10.1073/pnas.1703601115
发表时间：
2018-01-09
影响因子：
11.1
作者：
Li, Xiangning;Yu, Bin;Qiu, Zilong
通讯作者：
Qiu, Zilong