Sequential contribution of L- and P-selectin to leukocyte rolling in vivo.
Sequential contribution of L- and P-selectin to leukocyte rolling in vivo.
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DOI：
10.1084/jem.181.2.669
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发表时间：
1995-02-01
影响因子：
15.3
通讯作者：
Beaudet, Arthur L.
中科院分区：
文献类型：
--
作者：
Ley, Klaus;Bullard, Dan C.;Arbones, Maria L.;Bosse, Roland;Vestweber, Dietmar;Tedder, Thomas F.;Beaudet, Arthur L.
白细胞重新聚集到炎症部位是由与内皮细胞可逆的短暂黏附接触启动的，被称为白细胞滚动，这被认为是由选择素家族黏附分子介导的。选择素介导的滚动先于炎症细胞的迁移，后者在P-和L-选择素基因缺陷小鼠中都显著受损。我们在这里报道，在手术解剖后20分钟，大约有13%的白细胞通过野生型小鼠的提睾肌小静脉沿内皮细胞滚动。滚动式白细胞流量分数在40-60分钟达到最大值28%，80-120分钟恢复到13%。在P-选择素缺陷小鼠中，最初没有滚动，在80-120分钟时达到5%。L-选择素缺陷小鼠的滚动通量分数最初与野生型相似，80-120min时降至5%。在野生型和L-选择素缺陷小鼠中，P-选择素单抗RB40.34可完全阻断白细胞的初始滚动(0-60min)，但不受L-选择素单抗MEL-14的影响。相反，在较晚的时间点(60-120分钟)滚动被单抗Mel-14抑制，但不被单抗RB40.34抑制。经肿瘤坏死因子-α处理2小时后，野生型和P-选择素基因缺陷小鼠的所有通过的白细胞中约有24%在提睾主静脉中滚动。P-选择素单抗或E-选择素单抗10E9.6不影响肿瘤坏死因子-α处理的小鼠的滚动。相比之下，L-选择素单抗完全阻断了肿瘤坏死因子-α治疗的P-选择素缺陷小鼠的滚动。这些数据表明，P-选择素在组织创伤后白细胞滚动的最初诱导过程中起重要作用。在以后的时间点和经肿瘤坏死因子-α处理的制剂中，滚动在很大程度上依赖于L-选择素。在测试的条件下，我们无法找到E-选择素参与小鼠白细胞滚动的证据。
Leukocyte recruitment into inflammatory sites is initiated by a reversible transient adhesive contact with the endothelium called leukocyte rolling, which is thought to be mediated by the selectin family of adhesion molecules. Selectin-mediated rolling precedes inflammatory cell emigration, which is significantly impaired in both P- and L-selectin gene-deficient mice. We report here that approximately 13% of all leukocytes passing venules of the cremaster muscle of wild- type mice roll along the endothelium at < 20 min after surgical dissection. Rolling leukocyte flux fraction reaches a maximum of 28% at 40-60 min and returns to 13% at 80-120 min. In P-selectin-deficient mice, rolling is absent initially and reaches 5% at 80-120 min. Rolling flux fraction in L-selectin-deficient mice is similar to wild type initially and declines to 5% at 80-120 min. In both wild-type and L- selectin-deficient mice, initial leukocyte rolling (0-60 min) is completely blocked by the P-selectin monoclonal antibody (mAb) RB40.34, but unaffected by L-selectin mAb MEL-14. Conversely, rolling at later time points (60-120 min) is inhibited by mAb MEL-14 but not by mAb RB40.34. After treatment with tumor necrosis factor (TNF)-alpha for 2 h, approximately 24% of all passing leukocytes roll in cremaster venules of wild-type and P-selectin gene-deficient mice. Rolling in TNF- alpha-treated mice is unaffected by P-selectin mAb or E-selectin mAb 10E9.6. By contrast, rolling in TNF-alpha-treated P-selectin-deficient mice is completely blocked by L-selectin mAb. These data show that P- selectin is important during the initial induction of leukocyte rolling after tissue trauma. At later time points and in TNF-alpha-treated preparations, rolling is largely L-selectin dependent. Under the conditions tested, we are unable to find evidence for involvement of E- selectin in leukocyte rolling in mice.
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影响因子：
64.8
作者：
GALLATIN, WM;WEISSMAN, IL;BUTCHER, EC
通讯作者：
BUTCHER, EC
影响因子：
32.4
作者：
LAWRENCE, MB;BAINTON, DF;SPRINGER, TA
通讯作者：
SPRINGER, TA
DOI：
10.1083/jcb.121.2.449
发表时间：
1993-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Levinovitz A;Mühlhoff J;Isenmann S;Vestweber D
通讯作者：
Vestweber D
影响因子：
56.9
作者：
BEVILACQUA, MP;STENGELIN, S;SEED, B
通讯作者：
SEED, B
影响因子：
5.5
作者：
ATHERTON, A;BORN, GVR
通讯作者：
BORN, GVR