d-Leucine protects oocytes from chronic psychological stress in mice.
d-Leucine protects oocytes from chronic psychological stress in mice.
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DOI：
10.1002/rmb2.12396
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发表时间：
2021-10
影响因子：
3.4
通讯作者：
Matsuda S
中科院分区：
文献类型：
--
作者：
Tsuji A;Ikeda Y;Murakami M;Kitagishi Y;Matsuda S
心理压力对女性生殖能力有负面影响。d -亮氨酸（d - Leu）是一种存在于食物和哺乳动物组织中的d型氨基酸。我们研究了d - Leu对心理应激诱导的卵母细胞异常的保护作用。雌性小鼠（6周龄）分为三组：对照组、约束应激组（RS）和RS/d - Leu组。RS和RS/d‐Leu小鼠每天打孔3小时，共14天。RS/d‐Leu小鼠饲喂0.3% d‐Leu日粮。通过纺锤体和染色体的形状分析卵母细胞成熟失败。此外，我们还检测了卵巢中血红素加氧酶- 1 （HO - 1）和超氧化物歧化酶（SOD）的表达水平。d‐Leu是否会减少培养细胞中活性氧（ROS）的产生，我们用d‐Leu处理K562细胞，然后分析K562细胞中的ROS。RS小鼠卵母细胞成熟失败增加。d‐Leu将异常卵母细胞降低到控制水平。与RS小鼠相比，RS/d‐Leu小鼠HO‐1和SOD2的表达水平升高。添加d - Leu后，K562细胞中ROS水平呈剂量依赖性降低。我们得出结论，d‐Leu通过诱导HO‐1和SOD2表达，然后通过降低氧化应激来保护卵母细胞免受心理应激。d‐Leucine （d‐Leu）是一种d‐型氨基酸。d‐Leu降低了小鼠慢性心理应激诱导的卵母细胞成熟衰竭，并增加了小鼠卵巢中抗炎/抗氧化因子HO‐1和SOD2。
Psychological stress could negatively influence female reproductive ability. d‐Leucine (d‐Leu) is a d‐type amino acid found in foods and mammalian tissues. We have examined the protective effects of d‐Leu on oocyte abnormality induced by psychological stress. Female mice (6‐week‐old) were divided into three groups: control, restraint stress (RS), and RS/d‐Leu. The RS and RS/d‐Leu mice were holed for 3 hours daily during 14 days. RS/d‐Leu mice were fed 0.3% d‐Leu diet. The oocyte maturation failure was analyzed by shapes of spindles and chromosomes. In addition, levels of heme‐oxygenase‐1 (HO‐1) and superoxide dismutase (SOD) expression in the ovaries were also examined. Whether d‐Leu reduces the generation of reactive oxygen species (ROS) in cultured cells, K562 cells were treated with d‐Leu, and then ROS in K562 were analyzed. Oocyte maturation failure was increased in RS mice. d‐Leu reduced abnormal oocytes to control level. The expression levels of HO‐1 and SOD2 increased in RS/d‐Leu mice compared to those of RS mice. ROS levels were decreased in K562 cells with d‐Leu in a dose‐dependent manner. We concluded that d‐Leu protects oocytes from psychological stress through the induction of HO‐1 and SOD2 expression then by reducing oxidative stress. d‐Leucine (d‐Leu) is a d‐Type amino acid. d‐Leu reduced oocyte maturation failure induced by chronic psychological stress in mice, and d‐Leu increased HO‐1 and SOD2 that are anti‐inflammatory/anti‐oxidative factor in mice ovaries.
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影响因子：
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通讯作者：
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