MicroRNAs influence reproductive responses by females to male sex peptide in Drosophila melanogaster.
MicroRNAs influence reproductive responses by females to male sex peptide in Drosophila melanogaster.
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DOI：
10.1534/genetics.114.167320
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发表时间：
2014-12
期刊：
影响因子：
3.3
通讯作者：
Chapman T
中科院分区：
文献类型：
--
作者：
Fricke C;Green D;Smith D;Dalmay T;Chapman T
在整个分类群中，雌性的行为和生理学在交配过程中接受射精分子后发生了显着变化。例如，雌性黑腹果蝇（Drosophila melanogaster）接受性肽（SP）会显著改变雌性接受性、产卵量、寿命、激素水平、免疫力、睡眠和进食模式。这些变化是由不同的组织和时间特异性变化的不同套的mRNA。然而，很少有人知道这些基因表达变化的调节，因此microRNAs（miRNAs）的潜在作用，在女性交配后的反应。对女性接受SP的基因组反应的初步筛选表明，几种miRNA的表达发生了变化。在这里，我们直接测试了缺乏四种突出显示的候选miRNA（miR-279、miR-317、miR-278和miR-184）的雌性动物在与SP缺失或对照雄性动物交配一次或连续交配时，是否表现出对接受SP的生殖力、接受性和寿命反应的改变。结果表明，与对照组相比，缺乏miRNA的雌性与SP缺失的雄性交配后表现出接受性改变，但生殖输出没有改变。然而，这些影响与缺乏miRNA的女性的遗传背景显著相互作用。在连续与SP无效或对照雄性饲养的缺乏miRNA的雌性中未观察到显著的存活影响。然而，连续暴露于控制男性转移SP导致显着更高的变化，在miRNA缺乏女性的寿命比连续暴露于SP空男性。这些结果首次揭示了miRNAs在调节雌性交配后反应中的作用和重要性。
Across taxa, female behavior and physiology change significantly following the receipt of ejaculate molecules during mating. For example, receipt of sex peptide (SP) in female Drosophila melanogaster significantly alters female receptivity, egg production, lifespan, hormone levels, immunity, sleep, and feeding patterns. These changes are underpinned by distinct tissue- and time-specific changes in diverse sets of mRNAs. However, little is yet known about the regulation of these gene expression changes, and hence the potential role of microRNAs (miRNAs), in female postmating responses. A preliminary screen of genomic responses in females to receipt of SP suggested that there were changes in the expression of several miRNAs. Here we tested directly whether females lacking four of the candidate miRNAs highlighted (miR-279, miR-317, miR-278, and miR-184) showed altered fecundity, receptivity, and lifespan responses to receipt of SP, when mated once or continually to SP null or control males. The results showed that miRNA-lacking females mated to SP null males exhibited altered receptivity, but not reproductive output, in comparison to controls. However, these effects interacted significantly with the genetic background of the miRNA-lacking females. No significant survival effects were observed in miRNA-lacking females housed continually with SP null or control males. However, continual exposure to control males that transferred SP resulted in significantly higher variation in miRNA-lacking female lifespan than did continual exposure to SP null males. The results provide the first insight into the effects and importance of miRNAs in regulating postmating responses in females.
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影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
DOI：
10.1073/pnas.92.22.10114
发表时间：
1995-10-24
影响因子：
11.1
作者：
HERNDON, LA;WOLFNER, MF
通讯作者：
WOLFNER, MF
影响因子：
9.2
作者：
Carvalho, GB;Kapahi, P;Benzer, S
通讯作者：
Benzer, S
影响因子：
2.1
作者：
Fricke, C.;Bretman, A.;Chapman, T.
通讯作者：
Chapman, T.
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.