Gene expression profiling of reactive oxygen species (ROS) and antioxidant defense system following Sugarcane mosaic virus (SCMV) infection.
Gene expression profiling of reactive oxygen species (ROS) and antioxidant defense system following Sugarcane mosaic virus (SCMV) infection.
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甘蔗花叶病毒（SCMV）感染后活性氧（ROS）和抗氧化防御系统的基因表达谱。
DOI：
10.1186/s12870-020-02737-1
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发表时间：
2020-11-23
影响因子：
5.3
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Akbar S;Wei Y;Yuan Y;Khan MT;Qin L;Powell CA;Chen B;Zhang M
病毒是传染性病原体，植物病毒流行可能对作物产量和质量造成毁灭性后果。甘蔗花叶病毒属马铃薯Y病毒科，是每年危害甘蔗的主要病原之一。为了对抗病原体的攻击，植物产生活性氧物种(ROS)作为第一道防线，其复杂的平衡是通过良好组织的抗氧化剂清除途径实现的。本研究以甘蔗基因组为参照基因组，研究了甘蔗抗病(B-48)和感病(Badila)两个甘蔗品种ROS相关转录水平和ROS解毒途径的变化。转录组数据表明，在SCMV致病后，Badila基因型的ROS产生基因如NADH氧化酶、苹果酸脱氢酶和黄素结合单加氧酶显著上调。为了清除ROS，Badila基因的抗氧化剂，即谷胱甘肽S转移酶(GST)，与抗病基因相比有显著的增强。GST被认为是病原体攻击植物的关键指标。与Badila相比，B-48中GST的表达显著降低，这表明该基因具有抗性。此外，我们还鉴定了与赋予对B-48抗性有关的关键转录因子。其中，WRKY、AP2、NAC、bZIP和bHLH在B-48型中表达增强。我们的结果也证实了转录数据与酶和qPCR数据之间的联系。超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶和苯丙氨酸解氨酶活性的测定支持转录本数据，并显示出B-48基因型较高的抗性。目前的研究支持B-48基因在SCMV感染下的有效性。此外，还提供了比较的转录学数据，以强调重要的转录因子对该基因产生抗性的作用。本研究为甘蔗防御机制的表达谱研究提供了更深入的知识。网上版载有补充材料，可在10.1186/s12870-020-02737-1查阅。
Viruses are infectious pathogens, and plant virus epidemics can have devastating consequences to crop yield and quality. Sugarcane mosaic virus (SCMV, belonging to family Potyviridae) is one of the leading pathogens that affect the sugarcane crop every year. To combat the pathogens’ attack, plants generate reactive oxygen species (ROS) as the first line of defense whose sophisticated balance is achieved through well-organized antioxidant scavenging pathways. In this study, we investigated the changes occurring at the transcriptomic level of ROS associated and ROS detoxification pathways of SCMV resistant (B-48) and susceptible (Badila) sugarcane genotypes, using Saccharum spontaneum L. genome assembly as a reference genome. Transcriptomic data highlighted the significant upregulation of ROS producing genes such as NADH oxidase, malate dehydrogenase and flavin-binding monooxygenase, in Badila genotype after SCMV pathogenicity. To scavenge the ROS, the Badila genotype illustrated a substantial enhancement of antioxidants i.e. glutathione s-transferase (GST), as compared to its resistant counterpart. GST is supposed to be a key indicator of pathogen attacks on the plant. A remarkably lower GST expression in B-48, as compared to Badila, indicated the development of resistance in this genotype. Additionally, we characterized the critical transcription factors (TFs) involved in endowing resistance to B-48. Among these, WRKY, AP2, NAC, bZIP, and bHLH showed enhanced expression in the B-48 genotype. Our results also confirmed the linkage of transcriptomic data with the enzymatic and qPCR data. The estimation of enzymatic activities for superoxide dismutase, catalase, ascorbate peroxidase, and phenylalanine ammonia-lyase supported the transcriptomic data and evinced higher resistance in B-48 genotype. The current study supported the efficiency of the B-48 genotype under SCMV infection. Moreover, comparative transcriptomic data has been presented to highlight the role of significant transcription factors conferring resistance to this genotype. This study provides an in-depth knowledge of the expression profiling of defense mechanisms in sugarcane. The online version contains supplementary material available at 10.1186/s12870-020-02737-1.
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