Circular RNA circKIF4A facilitates the malignant progression and suppresses ferroptosis by sponging miR-1231 and upregulating GPX4 in papillary thyroid cancer.
Circular RNA circKIF4A facilitates the malignant progression and suppresses ferroptosis by sponging miR-1231 and upregulating GPX4 in papillary thyroid cancer.
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DOI：
10.18632/aging.203172
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发表时间：
2021-06-21
期刊：
影响因子：
--
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Chen W;Fu J;Chen Y;Li Y;Ning L;Huang D;Yan S;Zhang Q
环状RNA（CircRNA）是一类非编码RNA。它们在调节恶性进展中的各种生物学过程中起重要作用。但目前还不清楚大多数circRNA在甲状腺乳头状瘤进展中的具体机制。在本研究中，我们探索了circKIF 4A，结果显示它在甲状腺乳头状癌中有高表达。通过CCK-8、transwell和小鼠异种移植实验探索circKIF 4A的功能。敲低circKIF 4A可抑制甲状腺乳头状细胞的生长和迁移。此外，进一步进行RIP测定和双荧光素酶载体报告基因测定。我们的研究结果表明，circKIF 4A通过海绵状miR-1231和上调GPX 4表达促进甲状腺乳头状肿瘤的恶性进展。总之，我们的研究证明，circKIF 4A-miR-1231-GPX 4轴通过竞争内源性RNA在癌症增殖和铁凋亡中起着至关重要的作用。因此，靶向circKIF 4A极有可能成为未来治疗甲状腺乳头状癌的潜在方法。
Circular RNAs (circRNAs) are one type of non-coding RNA. They act as important role in regulating various biological processes in the malignant progression. But we don’t clearly know the specific mechanism of the majority circRNAs in papillary thyroid tumor progression. In the current study, we explored circKIF4A and the result showed that it had high expression in papillary thyroid cancer. The functions of circKIF4A were explored by CCK-8, transwell, and mouse xenograft experiments. Knockdown of circKIF4A could suppress papillary thyroid cell growth and migration. In addition, RIP assays and dual luciferase vector reporter assays were further conducted. Our consequence showed circKIF4A facilitated the malignant progress of papillary thyroid tumor by sponging miR-1231 and upregulating GPX4 expression. In conclusion, our study proved that circKIF4A-miR-1231-GPX4 axis played a vital role in cancer proliferation and ferroptosis by competing endogenous RNAs. Therefore, targeting circKIF4A is very likely to be a potential method for treatment of papillary thyroid cancer in the future.
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