A Degeneration Gradient of Poplar Trees Contributes to the Taxonomic, Functional, and Resistome Diversity of Bacterial Communities in Rhizosphere Soils.
A Degeneration Gradient of Poplar Trees Contributes to the Taxonomic, Functional, and Resistome Diversity of Bacterial Communities in Rhizosphere Soils.
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杨树的退化梯度有助于根际土壤细菌群落的分类、功能和抗性多样性
DOI：
10.3390/ijms22073438
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发表时间：
2021-03-26
影响因子：
5.6
通讯作者：
Ma Y
中科院分区：
文献类型：
--
作者：
Liu J;He X;Sun J;Ma Y
与根相关的细菌群落影响寄主植物的健康和营养。然而，不同健康条件下微生物组的差异尚不清楚。在这里，我们测试了这样的假设：根际土壤微生物多样性和功能随着杨树的退化梯度而变化，重点是植物生长促进细菌（PGPB）和抗生素抗性基因。包括分类学研究、功能检测和 ARG（抗生素抗性基因）注释在内的综合宏基因组分析表明，有效钾 (AK) 与微生物多样性和功能相关。我们提出了几种微生物：慢生根瘤菌、鞘氨醇单胞菌、中生根瘤菌、诺卡氏菌、Variovorax、Gemmatimonadetes、根杆菌、足球菌、Candidatus Solibacter、酸杆菌和苯杆菌作为反映土壤肥力和植物健康的候选微生物。健康杨树根际中最高丰度的多药耐药基因和四种主要微生物耐药机制（抗生素外流、抗生素靶点保护、抗生素靶点改变和抗生素靶点替代）证实了土壤肥力与微生物活性之间的关系。这一结果表明，健康的根际土壤含有更高容量的微生物，并具有更复杂的微生物相互作用网络，可以促进植物生长并降低细胞内抗生素水平。我们的研究结果表明植物退化梯度与细菌群落之间存在相关性，并深入了解高周转微生物群落的作用以及潜在的 PGPB 作为林业土壤质量的实时指标，并证明了细菌群落所贡献的内部相互作用。
Bacterial communities associated with roots influence the health and nutrition of the host plant. However, the microbiome discrepancy are not well understood under different healthy conditions. Here, we tested the hypothesis that rhizosphere soil microbial diversity and function varies along a degeneration gradient of poplar, with a focus on plant growth promoting bacteria (PGPB) and antibiotic resistance genes. Comprehensive metagenomic analysis including taxonomic investigation, functional detection, and ARG (antibiotics resistance genes) annotation revealed that available potassium (AK) was correlated with microbial diversity and function. We proposed several microbes, Bradyrhizobium, Sphingomonas, Mesorhizobium, Nocardioides, Variovorax, Gemmatimonadetes, Rhizobacter, Pedosphaera, Candidatus Solibacter, Acidobacterium, and Phenylobacterium, as candidates to reflect the soil fertility and the plant health. The highest abundance of multidrug resistance genes and the four mainly microbial resistance mechanisms (antibiotic efflux, antibiotic target protection, antibiotic target alteration, and antibiotic target replacement) in healthy poplar rhizosphere, corroborated the relationship between soil fertility and microbial activity. This result suggested that healthy rhizosphere soil harbored microbes with a higher capacity and had more complex microbial interaction network to promote plant growing and reduce intracellular levels of antibiotics. Our findings suggested a correlation between the plant degeneration gradient and bacterial communities, and provided insight into the role of high-turnover microbial communities as well as potential PGPB as real-time indicators of forestry soil quality, and demonstrated the inner interaction contributed by the bacterial communities.
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影响因子：
27.5
作者：
Armengaud P;Breitling R;Amtmann A
通讯作者：
Amtmann A
影响因子：
9.8
作者：
Ibekwe, A. Mark;Ors, Selda;Suarez, Donald L.
通讯作者：
Suarez, Donald L.
影响因子：
2.6
作者：
Danielsen, L.;Thuermer, A.;Meinicke, P.;Buee, M.;Morin, E.;Martin, F.;Pilate, G.;Daniel, R.;Polle, A.;Reich, M.
通讯作者：
Reich, M.
影响因子：
11.6
作者：
Dai, Zhongmin;Su, Weiqin;Xu, Jianming
通讯作者：
Xu, Jianming
影响因子：
4.4
作者：
Gottel, Neil R.;Castro, Hector F.;Schadt, Christopher W.
通讯作者：
Schadt, Christopher W.